摘要
目的探讨生理状态和脂多糖(lipopolysaccharide,LPS)刺激下,杀菌渗透增强蛋白(murine bactericidal/permeability-increasing protein,muBPI)在小鼠组织器官和细胞中的表达情况。方法①实验小鼠分为3组,即LPS未刺激组、LPS刺激组和阴性对照组;②制备小鼠心、肝、脾、肺、肾、胸腺、睾丸、小肠组织切片和骨髓细胞、腹腔灌洗细胞、骨髓源树突状细胞涂片;③采用免疫组化方法检测muBPI在上述组织器官和细胞中的表达。结果①LPS未刺激组小鼠睾丸、小肠、肾脏组织切片和骨髓细胞、腹腔灌洗细胞、骨髓源树突状细胞涂片均呈黄色着染;LPS刺激后上述组织切片和细胞涂片呈棕黄或深棕色着染;阴性对照组呈现非特异微弱黄色背景着染;②LPS未刺激组小鼠肺、肝组织切片呈微弱黄色背景着染;LPS刺激后上述组织切片呈棕黄色着染;阴性对照呈微弱黄色背景着染;③其余器官组织切片在LPS刺激或未刺激组均呈现与阴性对照组相同的非特异微弱黄色背景着染。结论①生理状态下,小鼠睾丸、小肠、肾脏、骨髓细胞、腹腔灌洗细胞和骨髓源树突状细胞可组成性表达BPI蛋白,LPS刺激后BPI蛋白表达量显著增高;②生理状态下,小鼠肺、肝组织不表达BPI蛋白,LPS可诱导上述组织表达BPI蛋白;③其余组织器官在生理状态下和LPS刺激后均不表达BPI蛋白。
Objective The bactericidal/permeability-increasing（BPI） protein is an important protein expressed in some cells,which has bactericidal activity on gram-negative organisms.It is involved in the defense against gram-negative bacterial infections and binds to LPS with high affinity.Its expression profile in human tissues has been revealed by previous studies in our laboratory and other laboratories.But no reports about its expression profile in mouse,the most popularly used animal model were published.This study aimed to reveal the expression profile of BPI protein in various tissues from mice in physiological condition and by LPS stimulation.Methods BPI protein expression was detected with immunohistochemical staining using anti-muBPI polyclonal anti-serum.Pre-bleeding rabbit serum was served as negative control.Different tissues from mice treated with LPS（550 ng per mouse） and untreated mice were examined,including testis,small intestine,heart,liver,lung,spleen,kidney,bone marrow,peritoneal enterocoelia clysis（PECs） and bone marrow-derived dendritic cells（BMDCs）.Results ① Murine BPI protein is weakly expressed in testis,small intestine,kidney,bone marrow,PECs and BMDCs in control group（untreated with LPS）,but its expression increased dramatically after LPS stimulation;② The expression of BPI protein could not be detected in liver and lung in control mice,but could be detected after LPS stimulation;③ Expression of BPI protein could not be detected in the other examined tissues,no matter with or without LPS stimulation.Conclusion This study has successfully revealed the expression profile of BPI protein in some tissues from mice in physiological condition or by LPS stimulation.Our results showed that BPI protein expressed physiologically in some tissues and cells in mice,and its expression level could be increased or induced by LPS stimulation.
出处
《首都医科大学学报》
CAS
北大核心
2009年第5期671-676,共6页
Journal of Capital Medical University
基金
北京市自然科学基金(7082016)资助项目~~
关键词
脂多糖
杀菌/渗透增强蛋白
小鼠BPI抗血清
免疫组化
lipopolysaccharide
bactericidal/permeability-increasing
anti-muBPI polyclonal anti-serum
immunohistochemistry
