摘要
1982年,Drewry报道了红松(Pinus resinosa)染色体G-带。随后,国内外一些学者用不同的处理方法,在多种植物染色体上诱导出了G-带,带纹清晰,数目多,沿染色体全长分布。这说明Greilhuber关于植物染色体不存在G-带的假说是不可信的。杉木的G-显带研究很少。夏晓敏等采用HSAG分带法,初步显出了杉木的染色体G-带,但多数染色体带纹尚欠清晰,而且没有进行G-带染色体的组型分析。我们应用改良的ASG法,首次在杉木前中期和中期染色体上诱导出清晰的G-带,并进行了G-带染色体的组型分析。
G-bands in prometaphase and metaphase chromosomes of Cunninghamia lanceolata collected from Huitong county in Hunan province, Rongshui county in Guangxi Zhuang Autonomous Region, and Shaowu county in \Fujian province, were induced successfully by appling a improved ASG method. The stripes of bands were clear and in a large number,distributed along the whole length of chromosomes. Band stripes in homologous chromosomes were comparatively similar in numbers, distribution, and size. But the character of band patterns were different among non-homologous ones. There were articulate phenomena in distribution of band stripes. Each articulation separated by light stain area was mostly consisted of two band stripes which were adjacent closely. At the phase of karyokinesis, band stripe numbers and distribution in homologous provenances were roughly similar.
出处
《林业科学》
EI
CAS
CSCD
北大核心
1990年第5期452-456,共5页
Scientia Silvae Sinicae
基金
国家自然科学基金资助项目
关键词
杉木
染色体
G带
Cunninghamia lanceilata
Chromosome
G-banding
