摘要
目的探讨脑外伤后不同阶段的神经细胞凋亡与c-fos基因表达Fos蛋白的水平,探讨c-fos基因在颅脑创伤后凋亡的作用。方法以Feeney法制作大鼠颅脑外伤模型,分为实验组和假手术对照组,在处理后6、24、168小时处死动物,分别用免疫组化、TUNEL法测定脑组织Fos蛋白和神经细胞凋亡率。结果颅脑创伤后6小时及24小时大鼠脑外伤组损伤灶周围脑组织中,Fos蛋白较假手术组表达明显增高,其与神经细胞凋亡指数呈正相关;在慢性期168小时大鼠脑外伤组损伤灶周围脑组织中,Fos蛋白表达与神经细胞凋亡有所下降而仍高于假手术组。结论c-fos基因很可能参与了颅脑创伤后的细胞凋亡过程,阻断c-fos基因在颅脑创伤后的表达上调,可望能够抑制神经细胞凋亡。
Objective focused on the expression of c-los on different stages after traumatic brain injury,and moreover analysis of relationship of the expression of c-fos and apoptosis after TBI. Methods Sprague-Dawley rats were assigned randomly to TBI group and sham control group. Rats in the TBI group were subjected to severe brain injury following Feeney＇s method,and rats in sham control group were subjected to the same operation without brain injury. In each group animals are sacrificed 6h,24h, 168b after operation. Fos and neurocyte apoptosis rate are defined by immunohistologieal method and TUNEL accordingly. Results The expression of c-fos in TBI group is higher than that in sham control group 6h and 24h after operation with statistical differences,which correlates to neuron apoptosis rate positively. The expression of c-los and neruocyte apopto- sis in TBI group are lower than before but still higher than that in sham control group 168h after operation. Conclusion The increase on expression＇ of c-fos after TBI probably contributes to neurocyte apoptosis,and thus the intervention of this expression may decrease neurocyte apoptosis after TBI
出处
《浙江创伤外科》
2009年第5期432-433,共2页
Zhejiang Journal of Traumatic Surgery
