摘要
目的:观察柴胡皂甙D(saikosaponin-d,SSd)对二乙基亚硝胺(diethylnitrosamine,DEN)致大鼠肝癌免疫功能的影响,并探讨其与SSd抗肝肿瘤作用的关系.方法:清洁级♂SD大鼠90只,平均体质量248.18±12.32g,随机分为5组:模型组(n=20),对照组(n=10)及SSd大、中、小剂量治疗组(均n=20).除对照组给予等量生理盐水灌胃外,其余各组大鼠均给予2mg/LDEN灌胃,按体质量10mg/kg给药,每周5次,同时各治疗组每天给予不同浓度SSd(2.0、1.5、1.0mg/kg)腹腔注射,至16wk停药,麻醉后处死大鼠.HE染色观察实验大鼠肝组织病理学结构的改变,应用流式细胞仪测定大鼠外周血T淋巴细胞亚群(CD3+CD4+%、CD3+CD8+%及CD3+CD4+/CD3+CD8+比值).结果:SSd各治疗组大鼠癌结节数及灶的大小均小于模型组.镜下单纯造模组癌细胞呈多形性,异形性明显,Edmondson分级多数属于Ⅲ级;相反,SSd各干预组癌细胞分化程度高,异型性较低,分级多为Ⅰ-Ⅱ级;肝癌模型组与正常对照组T淋巴细胞亚群比较CD4+、CD4+/CD8+明显下降,CD8+上升,差异有统计学意义(33.56%±4.16%vs45.50%±4.03%;1.06±0.56vs1.93±0.28;30.62%±3.65%vs22.88%±3.15%;均P<0.05);而SSd各组与肝癌模型组T淋巴细胞亚群比较CD4+、CD4+/CD8+明显回升,CD8+下降,差异有统计学意义,尤其以SSd大剂量组明显(39.06%±3.98%vs33.56%±4.16%;1.55±0.29vs1.06±0.56;18.99%±3.09%vs30.62%±3.65%;均P<0.05).结论:SSd对DEN诱发大鼠肝癌形成有一定的免疫保护作用.
AIM： To investigate the immunoregulatory effects of saikosaponin-d （SSd） in rats with diethylnitrosamine （DEN）-induced experimental hepatocarcinoma and explore whether such immunoregulatory effects are associated with the antitumor effects of SSd. METHODS： Ninety Sprague-Dawley male rats,weighting 248.18 ± 12.32 g, were divided into five groups randomly： normal control group （n = 10）, model control group （n = 20） and three treatment groups （n = 20 in each group）. Except the normal control group, rats in other groups were administrated with 2 mg/L DEN at a dose of 10 mg/kg bodyweight five times one week for sixteen weeks. Meanwhile, rats in the treatment groups were injected intraperitoneally with SSd at different doses （1.0, 1.5 and 2.0 mg/kg, respectively） once daily for sixteen weeks. Rats were anesthetized and killed at the end of last week. Hematoxylin and eosin （HE） staining was used to examine the pathological changes in the liver. T lymphocyte subsets （CD3± CD4±%, CD3± CD8±% and CD3± CD4±/ CD3± CD8± ratio） were measured by flow cytometry. RESULTS： Both tumor volume and tumor number were significantly reduced in the three SSd treatment groups when compared with the model control group. According to criteria of Ed- mondson and Steiner, the majority of tumors in the model group were histologically classified as grade III while those in the three SSd treatment groups were classified as grades I-II. The tumors in the treatment groups were well differentiated while those in the model control group were poorly differentiated. Compared with the control group, the percentage of CD4±T lymphocytes and CD4±/CD8± ratio significantly decreased in the model control group while the percentage of CD8＋ T lymphocytes markedly increased （33.56% ± 4.16% vs 45.50% ± 4.03%, 1.06 ± 0.56 vs 1.93 ± 0.28 and 30.62% ± 3.65% vs 22.88% ± 3.15%, respective- ly; all P 〈 0.05）. In contrast, a significant increase in the percentage of CD4＋ T lymphocytes and CD4＋/CD8＋ ratio and a marked decrease in the percentage of CD8＋ T lymphocytes were noted in the three SSd treatment groups （especially in high-dose SSd treatment group） when compared with the model control group （39.06% ± 3.98% vs 33.56% ± 4.16%, 1.55 ± 0.29 vs 1.06 ± 0.56 and 18.99% ± 3.09% vs 30.62% ± 3.65%, respectively; all P 〈 0.05）. CONCLUSION： SSd has inhibitory effects on DEN-induced hepatocarcinogenesis in rats perhaps through exerting an immunoprotective effect.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第24期2451-2455,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30471982~~
关键词
柴胡皂甙D
肝细胞癌
二乙基亚硝胺
Saikosaponin-d
hepatocellular carcinoma
Diethylnitrosamine
