摘要
为探讨投菌法处理印染废水前后对鲫鱼特异性免疫功能的影响，将鲫鱼腹腔注射嗜水气单胞菌疫苗预免疫后置于１３％浓度印染废水（ｐＨ＝７）、投菌法处理后出水和对照清水中，于第２，４，６，８，１０，１４，１８，２２，２５，３５天每次每组取鱼５尾，取血，测定各项免疫指标，结果发现清水和出水组鱼血中白细胞数及血清溶菌酶含量升高，分别于第８，６天达高峰，然后下降至基线水平。但１３％废水组鱼血白细胞数第２天降低，以后再逐渐恢复；该组鱼血清溶菌酶含量有下降趋势，且始终低于另两组。此外，废水组鱼血清抗嗜水气单胞菌抗体效价于第６天（抗体出现时）低于出水组，以后各组间差别无显著性。表明印染废水明显抑制了鲫鱼的免疫应答，而处理后出水则无此毒性作用。
In order to explore the effects of printing and dyeing wastewater untreated and treated by bacterial biotechnological method on the specific immune response of Carassius auratus, the tested fish were preimmunized intraperitoneally with Aeromonas hydrophila vaccine then were separately placed into control clean water,untreated printing and dyeing wastewater (dilution ratio 13%,pH=7) and treated water 5 fish of every group were killed on the day 2,4,6,8,10,14,18,25,35 th to detect their immune indexes The results indicated that total leucocytic numbers of blood and serum lysozyme level in fish of clean water and treated water increased rapidly and reached their peaks on the day 8th and 6th respectively after vaccination,then reduced to baseline and maintatined for a period of time Leucocytic numbers in fish in untreated wastewater group decreased on day 2nd then increased gradully to normal,serum lysozyme level in this group had decreasing tendency and was lower than the other 2 groups from beginning to end However titer of anti A hydrophila antibody in serum of untreated water group was only lower than that of treated water group on the day 6th when it appeared After this antibody titers of these 3 groups had no significant defference It indicated that printing and dyeing wastewater inhibited immune response of Carassius auratus ,but water treated by bacterial biotechnological method had no this kind of toxic effect
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1998年第6期265-268,共4页
Journal of Environment and Health
关键词
印染废水
投菌生物法
鲫鱼
免疫应答
生物监测
Printing and dyeing wastewater Bacterial biotechnology Carassius auratus Aeromonas hydrophila Immune response
