摘要
目的：建立适用于测定实验动物红细胞乙酰胆碱酯酶（ＡＣｈＥ）活性的自动化动力学法。方法：根据Ｗｈｉｔａｋｅｒ法稍加修改，编制适用于自动生化仪的ＡＣｈＥ动力学法分析程序，并作方法学性能评价。结果：在本法条件下，酶反应吸光度和时间之间的线性可达≥５ｍｉｎ；ＡＣｈＥ活性在２１０Ｕ／Ｌ范围内，呈线性关系，最低检测限为≤１．５Ｕ／Ｌ；批内和批间ＣＶ分别为１．１％～１．７％和３．０％～４．５％；正常参考值（ｘ±ｓ，ｋＵ／ＬＲＢＣ）：恒河猴１１．９０±１．７４（ｎ＝１９），比格狗１．８１±０．４７（ｎ＝４５），ＳＤ大鼠０．９７±０．２０（ｎ＝３０）。结论：自动化动力学法测定红细胞ＡＣｈＥ简便，精密度高，尤其适用于大鼠和狗等动物红细胞低活性ＡＣｈＥ测定。
Objective: To establish an automated kinetic method suited to determine erythrocyte acetylcholinesterase (AChE) activity in experimental animals. Methods: An kinetic analysis applied on automated biochemical analyzer for erythrocyte AChE were programmed according to Whittaker′s method with slight modification, and it′s methodological characteristic was evaluated. Results: Under conditions of present method,the linearity between absorbance and time for the enzymatic reaction was up to ≥5 min; linear activity was up to 210 U/L with a low detectior limit of 1.5 U/L; reference values (±s) of erythrocyte AChE in normal adult rhesus monkeys, beagle dogs and Sprague Dawley rats were 11.90±1.74(n=19), 1.81±0.47(n=45) and 0.97±0.20(n=30) kU/LRBC, respectively. Conclusion: The automated kinetic method for erythrocyte AChE is simple, sensitive, and applicable for determining erythrocyte AChE in animals, especially those (such as rat and dog) in which erythrocyte has a low AChE activity.
出处
《军事医学科学院院刊》
CSCD
北大核心
1998年第4期293-296,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
乙酰胆碱酯酶
红细胞
动力学
生化分析
acetylcholinesterase
erythrocyte
automated biochemical analyzer
kinetics
