摘要
目的采用两肾一夹肾血管性高血压大鼠（２Ｋ１Ｃ－ＲＨＲ）模型，探讨Ⅰ、Ⅲ型胶原在心肌纤维化形成过程及卡托普利治疗时的变化。方法建立２Ｋ１Ｃ－ＲＨＲ模型并随机分组，高血压６周组探讨模型６周时心肌胶原纤维的变化，高血压１２周探讨１２周时情况，卡托普利组治疗组６周组胶原纤维的改变。对照组为相应的假手术组。ＶａｎＧｉｅｓｏｎ染色和计算机图象分析作心肌胶原总定量测定。免疫组化作Ⅰ、Ⅲ型胶原半定量分析。结果高血压６周组和１２周组胶原总量增加，６周组以Ⅰ型胶原为主，１２周Ⅲ型胶原亦增加。治疗６周组Ⅰ型胶原减少，总胶原量亦减少，Ⅲ型胶原无显著变化。结论高血压组以Ⅰ型胶原增加为主，Ⅲ型胶原出现在后几周。卡托普利治疗６周有减少Ⅰ型胶原形成作用，但对Ⅲ型胶原作用不明显。
Aim\ To investigate the changes of type Ⅰ、Ⅲ collagen in 2K1C renovascular hypertensive rats(RHR) and the effect of captopril treatment.\ Methods\ 2K1C RHR were categoried into 6 weeks hypertensive group,12 weeks hypertensive group and captopril group. The respective 6 and 12 weeks sham operation group were used as control. Van Gieson staining and computerized image analysis system for total collagen analysis,collagan type Ⅰ、Ⅲ were immunohistochemical staining for differentiation.\ Results\ Total collagen and type Ⅰ collagen were significantly higher in hypertensive rats than control. The collagen type Ⅲ was significantly incraesed in 12 weeks, In captopril treatment group the type Ⅰ collagen was normalized but type Ⅲ collagen was not decreased. So the total collagen was lower than the untreated group but still higher than controls.\ Conclusion\ Myocardial fibrosis occured in 2K1CRHR in the early stage may be the prominent in the increasing of collagen type Ⅰ,but in the later time the type Ⅲ collagen is also reponsible of incraesing of total collagen. Captopril treatment decreased the type Ⅰ collagen, but had little effect on type Ⅲ collagen.
出处
《高血压杂志》
CSCD
1998年第4期310-312,共3页
Chinese Journal of Hypertension
关键词
肾血管性
高血压
心肌总胶原
卡托普利
治疗
myocardial collagen
\ 2K1C RHR
Van Gieson staining
\ Imunohistochemical staining
\ Ⅰ、Ⅲ type collagen
