摘要
本文研究低密度脂蛋白（ＬＤＬ）对内皮细胞表达促炎性细胞因子白细胞介素６（ＩＬ－６）和肿瘤坏死因子（ＴＮＦ）的影响及１７β－雌二醇的保护作用。以体外培养的人脐静脉内皮细胞（ＨＵＶＥＣ）为模型。将ＬＤＬ与ＨＵＶＥＣ孵育及ＨＵＶＥＣ经它莫西芬（Ｔａｍｏｘｉｆｅｎ）和／或１７β－雌二醇预处理后，再与ＬＤＬ孵育，检测培养液中ＩＬ－６和ＴＮＦ活性。发现ＬＤＬ可明显增加内皮细胞表达ＩＬ－６和ＴＮＦ。ＬＤＬ１００μｇ／ｍｌ时，ＴＮＦ为０．７４±０．０９（ｎｇ／ｍｌ），ＩＬ－６为９１６４±７３９（ｕ／ｍｌ），与对照组相比Ｐ＜００１；经１７β雌二醇预处理后，细胞在ＬＤＬ作用下，ＩＬ－６和ＴＮＦ活性与对照组相比无显著差异（Ｐ＞００５）；它莫西芬和１７β－雌二醇处理后，再加ＬＤＬ，ＩＬ－６和ＴＮＦ活性增高，与对照组相比有显著差异。实验结果表明：ＬＤＬ可促进血管内皮细胞高表达促炎性细胞因子ＩＬ－６和ＴＮＦ。雌激素可明显抑制ＬＤＬ引起的内皮细胞高表达ＩＬ－６和ＴＮＦ，从而产生对内皮细胞的保护作用。此作用可被它莫西芬拮抗。因此雌激素保护内皮细胞的作用，可能部分通过内皮细胞上雌激素受体。
A study on the effects of 17β-estradiol against the expression of interleukin-6 (IL-6) and tumor necrosis factor (TNF) induced by low-density lipoproteins(LDL) on vascular endothelial cells (VECs) was carried out. The level of IL-6 and TNF were measured in cultured human umbilical vein endothelial cells. LDL significantly raise the level of IL-6 and TNF in cultured endothelial cells. In LDL 100μg/ml, TNF activity was 0.74±0.09(ng/ml) and IL-6 activity was 91.64±7.39(u/ml).17β-estradiol can significantly decrease the expression of IL-6 and TNF induced by LDL in cultured endothelial cells. However, when tamoxifen and 17β-estradiol were added at the same time, the level of IL-6 and TNF significantly increased compared with that in the control group (P<0.05). These indicate that the protection of 17β-estradiol on VECs may be through estrogen receptor partially.
出处
《癌变·畸变·突变》
CAS
CSCD
1998年第6期352-355,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
内皮细胞
雌激素
LDL
IL-6
TNF
冠心病
endothelial cells
estrogen
low-density lipoproteins
interleukin-6
tumor necrosis factor
