摘要
实验使用的人成纤维细胞谱系ＭＳＵ－１包括由ｖ－ｍｙｃ转染正常二倍体人成纤维细胞及随后的自发遗传改变而形成的有不同程度生长优势的３个连续细胞株。这些衍生细胞株可能代表着由正常细胞向恶性细胞多步骤进展过程中不同的癌前阶段。使用致癌物诱发恶性转化以鉴别其不同类型细胞的反应，从而建立人类成纤维细胞肿瘤进展的体外模型。结果ＭＳＵ－１．１细胞（可能代表肿瘤进展早期阶段）在接触ＢＰＤＥ和１ＣＲ－１９１后形成转化灶，ＭＳＵ－１．２细胞（可能代表晚期阶段）经６０Ｃｏ照射后在软琼脂中形成高比例的大集落。自转化灶和软琼脂集落分离的细胞在裸小鼠体内形成持续性生长的纤维肉瘤。其亲代细胞ＭＳＵ－１．０不能被恶性转化。这一体外模型体系有助于阐明环境致癌的多步骤过程。
The MSU-1 lineage of human fibroblast including 3 immortalized cell strains with growth advantage to varying extents, was generated by transfection of normal human fibroblasts with a v-myc oncogene and their gaining additional genetic alteration(s) spontaneously. Those derived cell variants may represent different premalignant stages in the progression from normal cells to malignant cells. To establish in vitro model of human cell carcinogenesis, the 3 cell types were characterized for their responses to induction of malignant transformation by different carcinogens. The results shwoed that cell strains MSU-1.1 and MSU-1.2 were malignantly transformed after exposure to BPDE,ICR-191 and 60 Co γ-ray, respectively, followed by selection for focus forming ability or anchorage independent growth. The parental cell of the lineage MSU-1.0 was not found to be malignantly tranformed. Studies using this in vitro model with carcinogens revealed that different phenotypic properties of the resulted malignant cells were acquired by a step-wise progressive process.
出处
《癌变·畸变·突变》
CAS
CSCD
1998年第6期336-340,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
卫生部科研启动基金
关键词
成纤维细胞
化学致癌物
辐射
恶性转化
环境致癌
Human Fibroblast
Chemical Carcinogen
Radiation
Malignant Transformation
