摘要
对壳聚糖为壁材的共价交联固定、海藻酸钠为壁材的包埋固定及壳聚糖和海藻酸钠共用的新型复合载体(ACA)固定的β-葡萄糖苷酶进行了对比研究,得出ACA微胶囊固定化的酶具有较好的催化活性、重复使用和低温贮藏性能。醋酸和戊二醛及CaCl2的浓度、壳聚糖和海藻酸钠用量、引发和交联时间对ACA微胶囊固定酶的形态和性能影响较大,葡萄糖转化率随醋酸与戊二醛和CaCl2浓度的增加、壳聚糖和海藻酸钠用量的增大、交联和引发时间的延长呈先增大后减小的变化趋势,并于不同特定值达到最大。
The comparison study among β-glucosidase immobilized with chitosan by covalent cross-linking, with sodium alginate by embedded, or with the microcapsule containing both of them （alginate-chitosan-alginate, ACA） are made. The experimental results show that ACA microcapsule immobilized enzyme has better catalytic activity, reusability and the storage performance at low temperature. The concentration of acetate, glutaraldehyde and CaCl2 and the amount of chitosan and sodium alginate,the initiation and cross-linking time have big influence to form and properties of ACA microcapsule immobilized enzyme. The conversion rate of glucose first has a increase and then decreases with increase of the concentration of acetate, glutaraldehyde and CaCl2, the amount of chitosan and sodium alginate and the extension of initiation and cross-linking time, and reach the maximum by specific value respectively.
出处
《现代化工》
CAS
CSCD
北大核心
2009年第10期51-54,共4页
Modern Chemical Industry
基金
吉林省科技厅基础研究项目(20070577)
