摘要
为了从脑组织神经肽Ｙ水平探索运动性疲劳产生及药物调节的机理，采用ＡＢＣ免疫组织化学法结合图象分析，观察中药复方“体复康”对运动性疲劳大鼠不同脑区神经肽Ｙ的动态变化。结果显示：一定强度（速度由１５ｍ／ｍｉｎ递增至３５ｍ／ｍｉｎ，运动时间为２０至２５ｍｉｎ／ｄ）长期（共７周）运动的大鼠，安静状态下在丘脑室旁核（ＰＶ）、下丘脑背内侧核（ＤＭ）、下丘脑腹内侧核（ＶＭＨ）等核团ＮＰＹ无显著性变化；在此基础上的未次急性运动结束后３小时ＮＰＹ变化尤为明显。中药复方“体复康”对于慢性运动应激过程中ＮＰＹ的调节作用不明显，在急性运动应激中，中药复方对ＮＰＹ的调节在运动结束后３小时尤为明显，且呈双向调节，即在ＰＶ、ＤＭ核团下调，而在ＶＭＨ核团上调，结论：神经肽Ｙ在运动性疲劳大鼠不同脑区呈现不同的动态变化趋势，中药复方“体复康”对ＮＰＹ有双向调节作用。
The mechnism of exercise-induced fatigue(FIF)and the effect of "Tifukang" Recipe(TFK)on neuropeptide Y(NPY)of brainin rat with EIF were studied.Avidin-biotin-complex immunohistochemistry and image pattern analysis were used to observe the effect of TEK on the changes of NPY in different areas of brain.Results:After long term(7 weeks)and high intensity exercise (from15m/min gradually increased to 35m/min,20 to 25min/d),at rest,NPY was unchanged in paraventricularthalamic (PV),dorso- medial hypothalamic(DM)nucleus and ventromedial hypothalamic (VMH)nuclues,however,at 3 houre postexercise after final ex- ercise it changed obviously.NPY was not remarkably affected in the chronic fatigue stress by TFk,but it was regulated bidirection- ally by TFk,expecially at 3 hours post-exercise in the acute fatigue stress;down in PV and DM,while up in VMH.Conclusion;The dynamic changes of NPY in different areas of brain in rat with EIF were different,and TFK had bidirectionally regulation effect on NPY.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
1998年第4期312-315,共4页
Chinese Journal of Sports Medicine
基金
国家自然科学基金
关键词
中药复方
体复康
运动性疲劳
神经肽Y
Tifukang" Recipe
exercise-induced fatigue
neuropeptide Y
immunohistochemistry
