摘要
为探讨生长因子在浅Ⅱ度烫伤愈合中的作用提供基础资料，从分子水平研究了烫伤愈合渗出液细胞中生长因子ｍＲＮＡ的表达动态。在ＳＤ大鼠背部埋入海绵，于热烫伤浅Ⅱ度后６ｈ和１、３、７、１０、１４ｄ分别取渗出液细胞，提取细胞总ＲＮＡ，用逆转录聚合酶链反应方法，以βａｃｔｉｎ为内对照，计算ＴＧＦα、ＴＧＦβ１、ＰＤＧＦ和ｂＦＧＦｍＲＮＡ表达量的变化。结果表明：伤后６ｈ，ＴＧＦα、ＴＧＦβ１、ｂＦＧＦ均有表达；伤后３ｄＴＧＦα、ＴＧＦβ１、ＰＤＧＦ均出现表达峰，表达水平明显高于对照组（Ｐ＜０．０５）；伤后１０ｄＴＧＦβ１、ｂＦＧＦ出现表达峰，表达水平也明显高于对照组（Ｐ＜０．０５）。这提示它们在烫伤愈合的不同时期协同发挥效应。
he models of partial thickness burn wound were prepared with SD rats.The mRNA expressions of 4 growth factors in the exudate cells were analysed quantitatively with RTPCR method during the wound healing.The results showed that the expressions of TGFα,TGFβ1 and bFGF were observed at 6h after scald,the expression peaks of TGFα,TGFβ1 and PDGF were observed 3d after scald,the expression peaks of TGFβ1 and bFGF were observed 10d after scald,and the levels of these peaks were significantly higher than those in the control group (P<0.05).The results would be helpful for the further investigation of the mechanism of burn wound healing.
出处
《上海实验动物科学》
1998年第2期77-80,共4页
Shanghai Laboratory Animal Science
基金
国家自然科学基金
关键词
烫伤愈合
生长因子
MRNA表达
大鼠
Growth factor Burn wound healing Reverse transcriptionpolymerase chain reaction mRNA expression Rat
