摘要
为了优化毛云芝菌固体发酵培养条件,采用中心组合试验设计方法,研究初始加水量、接种量、培养温度及其交互作用对漆酶活力的影响。应用SAS软件和响应面分析相结合的方法,模拟得到回归方程的预测模型和可信度,并通过岭脊分析得到最佳的发酵条件为:装量为10 g干料的250 mL三角瓶中加蒸馏水38 mL,种子液4.3 mL,培养温度26.6℃。该条件下酶活达到1.4778×104U,约是优化培养条件前酶活的11.75倍。
In this manuscript, the environmental conditions for laccase production by Coriolus hirsutus solid substrate fermentation were carefully investigated through the central composite design. Those environmental conditions including ,the original humidity, inoculating amount and culture temperature. The predictive model and reliability were developed by SAS software and response surface analysis. The optimal fermentation condition were achieved and listed as follows： 38 mL distilled water,4.3 mL seed liquid of 250 mL flask with 10 g fresh substrate at the temperature of 26.6 ℃ through ridge analysis. With the optimum conditions, the laccase activity was achieved at 1. 4778× 10^4 U, was 11.75 times of that of the original condition.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2009年第5期688-692,共5页
Journal of Food Science and Biotechnology
基金
教育部留学回国科研启动基金(教外司留[2007]1108号)
关键词
毛云芝菌
漆酶
固体发酵
中心组合设计
岭脊分析
Coriolus hirsutus, laccase, solid substrate fermentation, central composite design, ridge analysis
