摘要
目的通过流式细胞仪对卵巢癌性腹水脱落细胞进行DNA倍体分析,并同血清CA125检测方法相比较,以评估二种方法及其联合检测在卵巢癌诊断中的价值。方法取2006年3月至2008年1月吉林大学第二医院妇产科及长春市结核病院卵巢癌患者腹水30例、结核性腹水15例。使用流式细胞仪对腹水脱落细胞进行DNA倍体分析,并同时检测血清CA125。结果卵巢癌性腹水组增殖指数(proliferation index,PI)、DNA指数(DNA in-dex,DI)均高于结核性腹水组,差异有统计学意义(P(0.05)。DNA倍体分析诊断卵巢癌的灵敏度为73.33%,特异度为93.33%;以血清CA125诊断卵巢癌的灵敏度为76.67%,特异度为26.67%。两种方法灵敏度差异无统计学意义(P(0.05),特异度差异有统计学意义(P(0.01)。结论使用流式细胞仪DNA倍体分析检测卵巢癌性腹水简单、快速且具有较高的灵敏度和特异度。如果联合血清CA125检测,对卵巢癌性腹水有更好的诊断价值。
Objective By flow cytometry DNA ploidy analyzing of cast-off cells in ovarian cancer ascites, and compared with serum CA125, to appraise the value of the two methods and their combination in ovarian cancer diagnosis. Methods Select 30 cases of ovarian cancer ascites as the experimental group, 15 cases of tuberculous ascites as the control group. The in-patients were in the department of gynecology and obstetrics of The Second Clinical Hospital of Jilin University and The TB Hospital of Changchun. Use flow cytometry to examine DNA ploidy analysis of cast-off cells in ascites. At the same time test these patients＇serum CA125. Result The PI, DI values in ascites of ovarian cancer were higher than those of tuberculous ascites, and there was significant difference （P 〈 0. 05 ）. The sensitivity of DNA ploidy analysis in ovarian cancer diagnosis was 73.33% and specificity was 93.33 %, the sensitivity of serum CA125 was 76. 67% and specificity was 26. 67% , their sensitivity had no significant difference （P 〉 0. 05）, but their specificity got a very significant difference （P 〈 0. 01 ）. Conclusion DNA ploidy analysis by flow cytometry is a simple and fast method to diagnose ovarian cancer and has high sensitivity and specificity. If it is combined with the serum CA125 ,ovarian cancer would be got a higher diagnostic value.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2009年第10期772-774,共3页
Chinese Journal of Practical Gynecology and Obstetrics
基金
吉林省科技厅资助课题(20030538-4)
