摘要
采用高脂饮食喂养兔制备动脉粥样硬化（ＡＳ）动物模型，测定血管环对去甲肾上腺素（ＮＥ）的收缩反应性，血管环ＣＧＭＰ浓度变化及其诱导型一氧化氮合酶（ｉＮＯＳ）活性的变化。结果表明：ＡＳ兔血管环对ＮＥ的反应性减弱（Ｐ＜０．０５），ＣＧＭＰ浓度减低（Ｐ＜０．０５），ｉＮＯＳ活性明显增强（Ｐ＜０．０５）；加入左旋精氨酸（Ｌ－Ａｒｇ）和硝普钠（ＳＮＰ）后ＡＳ兔血管环对ＮＥ的反应进一步降低（Ｐ＜０．０１），ＣＧＭＰ浓度增加（Ｐ＜０．０１）。一氧化氮合酶（ＮＯＳ）阻滞剂Ｌ－硝基精氨酸（Ｌ－ＮＮＡ）的作用则与上述相反（Ｐ＜０．０１）。
Atherosclerotic lesions contain vascular smooth cells and thus may have an increased capacity for generation of nitric oxide (NO) by inducible NO synthase (iNOS). Rabbits (n=8) were raised for eight weeks by the atherogenic diet, then the aortic rings were captured. Contractive responsiveness to norepinephrine, changes of CGMP, expression of iNOS of aortic rings were examined in vitro. There was a substantial decrease in contractile responsiveness to norepinephrine (NE) (P<0.05), a decrease of CGMP (P<0.01) and an increase of expression of iNOS (P<0.05), after Larginine or sodium nitroprusside treatment. The contractile responsiveness to NE was atenuated (P<0.01). The CGMP was increased ((P<0.01). After NGnitric arginine (LNNA) treatment, those reaction were reversed. It implied that Larginine/NO pathway obstructure might lyikely be another mechanism of atherosclerosis and atherosclerotic arteries and had an increased capacity for generation of NO by iNOS.
出处
《湖南医科大学学报》
CSCD
1998年第4期349-352,共4页
Bulletin of Hunan Medical University
关键词
一氧化氮
动脉粥样硬化
兔
主动脉
INOS
nitric oxide
enzymes
atherosclerosis
inducible nitric oxide synthase*
rabbits
