摘要
应用半套式PCR技术从Karp株恙虫病立克次体基因组中扩增出872hpDNA片段。该片段经Pstl消化后，与经PStl酶解的pUC18连接，转化大肠杆菌，电泳初筛得pUCRL3等重组质粒。pUCRL3经PCR及酶切证实含约294hp恙虫病立克次体sta56基因片段，可作为检测恙虫病立克次休的特异核酸探针。
From the genome of Rickettsia tsutsugamushi, strain Karp,an 872 --hp fragment of partial sta56 gene was amplified by semi--nested polymerase chain reaction. A 294--hp Pstl fragment deriving from the 872hp one was cloned into pUC18 yielding pUCRL3,the recombinant plasmid was confirmed by PCR using. R.tsutsugamushi specific primer and pUC/M13 forward sequencing primer. The probe can be used to detect. R.tsul-sugamushi DNA by nucleic acid Hybridization.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1998年第5期69-70,共2页
Chinese Journal of Zoonoses
