摘要
目的:研究妊娠妇女骨骼肌中葡萄糖转运蛋白4(glucose transporter 4,GLUT4)、磷酸化-一磷酸腺苷激活的蛋白激酶(Pro-AMP activated protein kinase,Pro-AMPK)的表达及其与妊娠期胰岛素抵抗的可能关系。方法:用超高速离心法分离骨骼肌细胞膜。用免疫印记(Western blot)检测15例正常妊娠妇女(产妇组)和13例正常未孕妇女(对照组)骨骼肌Pro-AMPK、细胞膜GLUT4蛋白的表达,同时检测体重指数(BMI)、血脂水平和胰岛素抵抗指数(HOMA-IR)。结果:(1)产妇组HOMA-IR的水平明显高于对照组(P=0.002);血清总胆固醇(TC)、甘油三酯(TG)水平差异有统计学意义(P<0.05),而高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平差异无统计学意义(P>0.05),但都在正常范围内。(2)产妇组骨骼肌AMPK磷酸化水平与细胞膜GLUT4蛋白表达均明显低于对照组,差异有统计学意义(P<0.0001)。(3)产妇组骨骼肌AMPK磷酸化程度及细胞膜GLUT4蛋白表达水平分别与HOMA-IR呈负相关(r=-0.547,-0.540;P=0.0347,0.0379)。GLUT4蛋白表达水平与AMPK磷酸化程度无相关性(r=0.243;P=0.3824)。结论:妊娠可能抑制葡萄糖利用的AMPK信号通路,降低AMPK活性及GLUT4细胞膜转位从而参与妊娠期胰岛素抵抗的发生。
Objective：To study the expression of GLUT4,Pro-AMPK in skeletal muscle cell in pregnant women and its possible relationship to gestational insulin resistance. Methods： The skeletal muscle cell membrane was abstracted by hypervelocity centrifugation. The expressions of Pro-AMPK and GLUT4 protein in skeletal muscle cell membrane were determined by Western blot in normal Pregnant （ pregnant group, n = 15 ） and non-pregnant women （ non-pregnant group,n = 13）. The body mass index （BMI）, serum fat and insulin resistance index （HOMA-IR） were also measured. Results：（ 1 ）The level of HOMA-IR of pregnant group was signifi- cantly higher than that in non-pregnant group （P = 0. 002）. The levels of TC, TG between the two groups had significant differences（ P〈0.05 ）, while the levels of HDL, LDL had no significant diiferences （ P〉0.05 ）. （2） The levels of Pro-AMPK and GLUT4 protein in skeletal muscle cell membrane in pregnant women were significantly lower than those in non-pregnant women （P 〈0. 0001 ）. （3）GLUT4 and Pro-AMPK protein both were negatively correlation to HOMA-IR（r =-0. 547, - 0. 540 ; P = 0. 0347,0. 0379 ）. There was no correlation between the levels of GLUT4 and Pro-AMPK protein （ r = 0. 243 ; P= 0. 3824 ）. Conclusion ： Gestation may AMPK signaling pathway; the AMPK activity decrease and the decrease of GLUT4 in skeletal muscle cell membrane maybe one of the molecular mechanisms leading to insulin resistance.
出处
《现代妇产科进展》
CSCD
北大核心
2009年第9期671-674,共4页
Progress in Obstetrics and Gynecology
关键词
GLUT4
磷酸化AMPK
胰岛素抗药性
骨骼肌细胞膜
Glucose transporter
Pro-AMPK
Gestational muscle cell membrane expression gestational resistance
Skeletal
