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不同深度烫伤大鼠创面组织中β_1整合素、整合素连接激酶的表达

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摘要 【目的】通过观察不同烫伤程度大鼠在烫伤24 h后β_1整合素和整合素连接激酶(ILK)的表达,探讨β_1整合素和ILK在创面愈合中的作用。【方法】将32只SD大鼠分别造成Ⅰ、浅Ⅱ、深Ⅱ度和Ⅲ度烫伤,伤后24 h取创面组织标本,制作切片,分别进行HE染色以及整合素β_1、ILK免疫组化染色,观察不同烫伤程度创面整合素β_1和ILK的表达与定位。【结果】Ⅰ度烫伤大鼠表皮基底层和皮肤附属器可见明显整合素β_1和ILK表达,其中ILK在皮肤附属器的表达较整合素β_1更为显著;浅Ⅱ度组织创面,残留的基底层和健全的皮肤附属器周围有整合素β_1和ILK表达,其中ILK在皮肤附属器的表达较整合素β_1更为显著,而整合素β_1主要在毛囊表达;深Ⅱ烫伤创面仅真皮深层健存的皮肤附属器中有整合素和ILK阳性细胞表达;Ⅲ度烫伤创面未见整合素β_1和ILK阳性。【结论】整合素β_1和ILK具有相似的局限表达模式并与大鼠烫伤程度密切相关,提示ILK可能介导整合素的胞内的信号通路,与整合素β_1共同参与创面愈合过程。
作者 雷俊霞 王立晖 谢明伟 潘姝 李叶扬
机构地区 中山大学中山医学院病理生理教研室 中山大学中山医学院 广州市红十字会医院
出处 《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2009年第A03期42-44,共3页 Journal of Sun Yat-Sen University:Medical Sciences
基金 广州市医药卫生科技重点项目(2006-zdi-08)
关键词 烫伤 创面愈合 Β1整合素 整合素连接激酶
分类号 R392.11 [医药卫生—免疫学] R644.02 [医药卫生—外科学]
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参考文献8

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共引文献29

中山大学学报（医学科学版）
2009年 第A03期

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