摘要
为了找出褪黑素分泌变化与c-fos原癌基因表达之间的关系,本研究控制不同光照时间饲养1日龄雏鸡50只,取骨髓组织,经免疫组化试验,结果显示:①单核细胞系胞浆出现阳性信号;巨核细胞系、粒细胞系和淋巴细胞系胞核出现阳性信号;红细胞系无阳性信号;②随着光照时间的延长,c-fos原癌基因的表达量呈增长趋势。结果表明,c-fos与骨髓细胞的细胞增殖和分化相关。褪黑素的增高能够显著抑制c-fos原癌基因的表达,且褪黑素的减少能够刺激c-fos的表达。
In order to find out the relationship between the changes of melatonin secretion and expression of c-fos oncogene, in this study, we have one day-old chicks by controlling different illumination conditions, then take out 1 cm bone marrow, immunohistochemical analysis showed that： （1）positive signal in the cytoplasm of monocytic series; positive signal in the cell nuclei of megakaryocytic series, granulocytic series and lymphocyteic series; no positive signal in erythrocyte. （2）With the extension of illumination time, the expression of c-fos had a growth trend. The results showed that c-fos is related to cell proliferation and differentiation of bone marrow cells. The increase of melatonin can significantly inhibit c-fos oncogene expression and the reduction of melatonin can stimulate c-fos expression. According to the results of this experiment can speculate that melatonin can impact on proliferation and differentiation of bone marrow cells by regulating the expression of c-fos.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第9期186-189,共4页
China Animal Husbandry & Veterinary Medicine
