摘要
以产酸克雷伯氏菌(Klebsiella oxytoca)M5al为出发菌株,经亚硝基胍诱变处理,运用质子自杀法选育,从含0.17mol/L NaBr-NaBrO3的初筛平板上选出44个具有稳定遗传性的单菌落,然后结合培养基优化后的摇瓶发酵复筛,获得3个产乳酸突变株,其乳酸脱氢酶活性分别为出发菌株的50.6%、58.8%、61.3%。对其中乳酸脱氢酶活性最低的菌株在5L自动发酵罐上进行批式发酵,结果显示:突变株乳酸产量大幅降低,而乙酸、1,3-丙二醇的产量则显著增加。
Klebsiella oxytoca M5al was used as the original strain to breed mutants after mutagenesis with NTG 44 single colony were obtained from medium containing 0.17 mol/L NaBr-NaBrO3 （proton suicide method） and 3 lactic acid-producing mutants were selected by shaking cultivation in optimized medium, their lactate dehydrogenase activity was only 50.6%, 58.8%, 61.3% of the parent strain, respectively. The lowest one of them batch-cultured in 5 L fermenter, results indicated that the yield of lactic acid of the mutant was decreased remarkably, reversely, acetic acid and 1,3-propanediol were increased observably.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第9期1368-1371,共4页
Microbiology China
关键词
质子自杀
产酸克雷伯氏菌
产乳酸突变株
乳酸脱氢酶
Proton suicide, Klebsiella oxytoca, Lactic acid-producing mutant, Lactate dehydrogenase
