摘要
本研究从秸秆中分离得到一株弹性蛋白酶高产菌,并对该菌株进行鉴定,以期为实现其工业化生产提供理论依据。采用酪蛋白(脱脂奶粉)进行初筛后,以弹性蛋白(牛筋)进行复筛,并对筛选结果进行检验,然后对所得菌株结合形态、生理生化特性和16SrRNA基因序列进行分类鉴定,得到一株弹性蛋白酶高产菌株LSF-97。结果显示,菌株LSF-97与短小芽孢杆菌同源率达到100%,形态及生理生化特征与模式菌也显示高度一致性,故将其确定为短小芽孢杆菌。
The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw, and the selected strain was identified. The screening strategy included casein （skim milk） plate selecting and elastin （beef tendon） re-screening. And then, the morphological, physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally, the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree, the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第9期1345-1349,共5页
Microbiology China
基金
国家863计划项目(No.2007AA021503)
教育部长江学者和创新团队发展计划资助(No.200558)
关键词
芽孢杆菌
蛋白酶
筛选
鉴定
Bacillus, Elastase, Screening, Identification
