摘要
霍乱的病原菌是Ｏ１群霍乱弧菌，但在Ｏ１群霍乱弧菌中，仅有部分可以引起霍乱流行，因此不同菌株在流行病学上的意义差异很大。为探讨Ｏ１群菌不同型别和亚群之间的遗传关系和进化变异规律，应用多位点酶电泳法对Ｏ１群霍乱弧菌进行研究。结果：Ｏ１群霍乱弧菌的大多基因位点等位基因频率较低，尤其是古典型菌株和埃尔托型流行株，大多被检测基因为非多态位点；Ｏ１群霍乱弧菌的平均遗传多态值（ＨＯ１）为０．１８３，平均杂合度（ｈＯ１）为５．６７；其中古典型菌、埃尔托型流行株、埃尔托型非流行株的平均遗传多态值和平均杂合度分别为：ＨＣＶＣ＝０．１０６，ｈＣＶＣ＝１．５；ＨＥＳＥＶＣ＝０．０５６，ｈＥＳＥＶＣ＝２．３；ＨＮＳＥＶＣ＝０．４２５，ｈＮＳＥＶＣ＝５。结果显示：古典型和埃尔托型流行株作为霍乱的病原体是一个遗传关系紧密的群体，而埃尔托型非流行株遗传关系较为离散，与流行株之间的关系也较远。
Vibrio cholerae serogroup O1 are considered to be the pathogen of cholera. By different typing methods, Vibrio cholerae O1 can be classified into many subgroups or subtypes. The significance of them on epidemiology are not same. In order to study the genetic relationships among the subgroups and their evolution characteristics we studied 471 isolates of Vibrio cholerae O1 with the method of multilocus enzyme electrophoresis (MEE). The results showed that the allele frequencies of most gene loci in Vibrio cholerae O1 were very low. Most of the gene loci detected were not diversified, especially in Classical strains and epidemigenic strains of El Tor. The average genetic diversity and heterozigosity in Vibrio cholerae O1(O1), Classical (CVC), Epidemigenic strains of El Tor (ESEVC), and Non epidemigenic strains of El Tor (NSEVC) were repectively as follow: H O1 =0.183,h O1 =6.33;H CVC =0.106,h CVC =1.5;H ESEVC =0.056, h ESEVC =2.3;H NSEVC =0.425, h NSEVC =5. The results indicated that CVC and ESEVC, as the pathogens of cholera, were very close in genetic relationships. Meanwhile the relationships in NSEVC, as the environmental strains, were disperse.
出处
《河南医科大学学报》
1998年第3期24-29,共6页
Journal of Henan Medical University
基金
国家自然科学基金
关键词
O1群
霍乱弧菌
群体遗传学
遗传多态度
杂合度
Vibrio cholerae O1
population genetics
genetic diversity
heterozigosity
