摘要
当人胚肺成纤维细胞被传至40代龄后,在培养液中加入人参根皂甙(SRG)sμg/ml,人参果皂甙(SFG)1.5μg/ml和人参茎叶皂甙(SSLG)1.0μg/ml可分别延长细胞传代寿命的120％,110％和130％。当培养液中三种皂甙的终浓度分别在1.78～16.00μg/ml,0.50～4.50μg/ml和0.25～4.00μg/ml范围内时。实验结果表明,高代龄细胞的饱和密度明显增加,细胞的克隆生长也受到显著影响,SRG的作用较SFG和SSLG更明显。而同样条件下三种皂甙对低代龄细胞的影响则不明显。
Saponins extracted from roots (SRG), fruits (SFG) and stems-leaves (SSLG) of Ginseng were added to the culture medium of human embryoniclung fibroblasts.The concertrations of SRG of 5μg/ml, SFG of 1.5μg/ml and SSLG of 1.0μg/ml were used and the populaion doublings were increased by 120%, 110% and 130%. When SRG,SFG and SSLG concertrations were 1.78～16.00μg/ml, 0.50～4.50μg/ml and 0.25～4.00μg/ml in the culture medium, there were increase of the clonal growth and the saturation density in old (40th passage) cultures, whereas no effect on young 27th passage) cultures,
出处
《老年学杂志》
CSCD
1990年第2期103-105,共3页
关键词
人参皂甙
纤维细胞
寿命
Human fibroblast
lifespan
Ginseng saponin
