摘要
目的制备兔抗猪纤维连接蛋白(FN)抗血清,并探讨不同免疫途径、免疫次数及兔品种对抗血清效价水平的影响。方法用猪血浆FN分别免疫纯种新西兰兔和杂种兔,将兔分别随机分成4组,即皮下注射1次及另加淋巴结注射5次组和3次组、肌肉注射接种1次及另加静脉注射5次组和3次组,用免疫双扩散法测定FN抗血清效价。结果两种免疫途径都可产生特异性抗血清。皮下+淋巴结途径抗血清效价明显高于肌肉+静脉注射(P<0.05),5次接种血清效价较3次高(P<0.05),新西兰兔血清效价高于杂种兔(P<0.05)。结论制备兔抗猪血浆FN血清,皮下+淋巴结注射和多次加强免疫是较好的免疫方法,纯种新西兰兔较杂种兔更易产生兔抗猪FN抗血清。
Objective To prepare Rabbit anti-pig plasma fibronectin （FN） serum and to explore the effect of different immune approaches, the schedule of immunization and species of rabbits on the antibody titer. Methods Inbred New Zealand rabbits and hybrid rabbits respectively were inoculated with different programs. Then, FN antibody was titrated by double immundiffusion. Results New Zealand rabbits and hybrid rabbits could bring anti-FN through hypodermic injection plus lymph nodethen injection or intramuscular injection plus intravenous injection. In addition, the antibody titer of the former was obviously higher than that of the latter and the 5 times booster immunization was higher than 3 times booster immunization. Conclusion To obtain rabbit anti-pig plasma FN serum, it is better to inoculate rabbits through hypodermic injection plus lymph nodethen injection with multiple immunization. Moreover, inbred New Zealand rabbits were easy to induce anti-FN than hybrid rabbits.
出处
《基础医学与临床》
CSCD
北大核心
2009年第9期970-973,共4页
Basic and Clinical Medicine
关键词
纤维连接蛋白
抗血清
制备
免疫途径
fibronectin
antiserum
preparation
route of immunization
