摘要
目的从新分离的枯草芽孢杆菌中克隆和鉴定蛋白酶基因。方法用PCR方法从新分离的枯草芽孢杆菌DNA扩增出蛋白酶基因,PCR产物克隆入pMD-18T载体,DNA序列分析鉴定重组的插入片段;DNA序列分析结果与GenBank上的序列进行比较确定新克隆蛋白酶基因的种类。结果从枯草芽孢杆菌DNA中扩增出大约1200bp的PCR产物,重组入pMD-18T载体,DNA序列分析结果显示插入的DNA片段全长1146个核苷酸,在Genbank搜索显示为枯草芽孢杆菌蛋白酶基因全序列,与GenBank报道的所有序列均有差别。结论从新分离的枯草芽孢杆菌DNA中克隆出新的蛋白酶基因。
Objective To clone and identify a protease gene from a novel Bacillus subtilis. Methods The protease gene was am- plified from DNA of a novel Bacillus subtilis by PCR. The PCR product was inserted into pMD-18T vector which was identified with DNA sequence. The results of DNA sequence was matched with the sequences in GenBank for identifying the gene. Results A fragment of PCR product about 1 200bp was amplified, and the PCR product was recombined into pMD-18T. Sequencing the recombinant vector shew that the inserted fragment was 1 146bp which was protease gene of Bacillus subtilis, but it was different from all of reported sequence in GenBank. Conclusions A new protease gene is cloned and identified from a novel Bacillus subtilis.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第17期2218-2220,共3页
Chinese Journal of Gerontology
基金
吉林省发展改革委员会基金(2005)
