摘要
采用ｐｅｒｃｏｌ梯度密度法和电泳法分离了奶牛的精液，并用分离后的冷冻精液给４６０头母牛输精。试验牛的产母率，ｐｅｒｃｏｌ梯度密度法为６３．５％，电泳法为６３．０７％，分别比对照组（４８．８％）提高１４．７％和１４．２７％，差异均极显著（ｐ＜０．０１，ｐ＜０．０１）。两种分离方法间对性控影响的差异不显著（ｐ＞０．０５），相比较，电泳法更适合奶牛性控生产中应用。
Percoll density method and electrophoresis method were used in dairy seman separated. The dairy was sepermaticed using the seperated seman.The percent of female calf for experimented dairy were 63.5% for percoll density method and 63.07% for electrophoresis method, 14.7% and 14.27% higher than that of contrast group respectively.The differences were significant ( P <0.01, P <0.01).However the differences between two methods of separate were not stotistically significant ( p >0.05).The electrophoresis method was more suitable than percoll density method in sex control of dairy.
基金
吉林省科委下达研究课题
关键词
性别控制
电泳法
分离精子
产母率
奶牛
sex contrcl, percoll density, electrophoresis method, seman separation, female calf percent
