摘要
考察了高山红景天致密愈伤组织颗粒（ＣＣＡ）在５Ｌ气升式反应器中进行悬浮培养的动力学特性，得到了生物量１３．２３ｇ／Ｌ，红景天甙含量０．３５４％；培养液几乎保持纯清，粘度变化很小，反应器内没有出现“发泡”现象。培养系统的传质特性较好，在整个培养过程中，体积氧传递系数ｋＬａ先呈增大趋势，后又降低。
The kinetics and oxygen transfer characteristics of suspension culture of Rhodiola sachalinensis compact callus aggregates (CCA) in a 5L air lift reactor were investigated. It was shown that a high biomass of 13.23g/L was reached, corresponding to the salidroside content of 0.354%. The culture broth almost retained clear throughtout the culture. As a result no “foaming” was found in the reactor. The mass transfer capacity of the culture system was good with the oxygen transfer coefficient k L a increasing in the early stage of cultures then dropping. It was confirmed that the significant increase in solid holdup of the culture system contributed to the variation of k L a during the whole culture period.
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
1998年第3期305-312,共8页
Chemical Reaction Engineering and Technology
基金
国家自然科学基金资助项目
关键词
高山红景天
悬浮培养
CCA
红景天甙
生产
Rhodiola sachalinensis
Callus
Suspension culture
Air lift reactor
Volumetric oxygen transfer coefficient
