摘要
目的：获得ＩＬ２／ＨＢＶｐｒｅＳ融合基因克隆，为表达ＩＬ２／ＨＢＶｐｒｅＳ融合蛋白奠定基础。方法：用ＰＣＲ方法从乙肝全序列中扩增ＨＢＶｐｒｅＳ基因片段，并克隆入带有ＩＬ２基因的ｐｌｙ５质粒中，挑出的阳性克隆再亚克隆到ｐＵＣ１８中进行序列测定。结果：基因全长９３０ｂｐ，编码３１０个氨基酸，序列内有起始码和终止码。结论：所克隆的基因为ＩＬ２与ＨＢＶｐｒｅＳ的融合基因。
Aim: To clone the IL 2/HBV preS fusion gene, for expression of IL 2/HBV preS fusion protein. Methods: With PCR technique, the whole gene of HBV preS was amplified and cloned into IL 2 ply5 plasmid vector. The IL 2/HBV preS fusion gene was characterized by nucleotide sequence analysis. Results: The amplification reaction yielded a HBV preS fragment of 525 base pairs. The fusion gene was 930 base pairs in whole length in which the initial codon and terminator were found and 310 amino acids can be encoded. Conclusion: The cloned gene is IL 2/HBV preS fusion gere.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1998年第1期29-32,共4页
Chinese Journal of Cellular and Molecular Immunology
