摘要
通过分子克隆技术将ＩＬ６基因导入大肠癌细胞ＨＴ２９，建立了目的基因转导株ＨＴ２９ＩＬ６细胞。该转导株接种于裸鼠皮下后，与非转导株相比，长出肿瘤的时间延后，最终瘤径明显较小，而且移植瘤标本镜下可见大量的核固缩、核染色质加深、核断裂等凋亡细胞。用携带ＩＬ６基因的重组逆转录病毒液感染大肠癌细胞Ｌｏｖｏ后，仅以１．２５μｍｏｌ／Ｌ的ＶＵＭＯＮ２６（简称ＶＭ２６）即可诱导该转染株发生明显凋亡，而非转染株用５μｍｏｌ／Ｌ浓度的ＶＭ２６方能奏效。可见，转导株对细胞凋亡诱导剂的敏感性明显增强。提示ＩＬ６基因表达可促进或诱导肿瘤细胞进入凋亡过程，从而抑制肿瘤的形成与发展。
Human interleukin 6(hIL 6) gene was transferred into human colon cancer cell line HT 29 through molecular cloning technique to develop the target gene transfected HT 29 IL 6 cell strain. The transfected cells were inoculated subcutaneously in nude mice. Compared with the control group, the tumor forming time was delayed, the final tumor size significantly reduced. The transfected tumor samples under a microscope showed a large amount of apoptosis cells characterized with karyopyknosis increased karyotin and karyclasis. Only 1.25 μmol/L VM 26 could induce apoptosis in the colon cancer cells Lovo transfected by recombinant virus suspension bearing IL 6 gene, while in the nontransfected group 5 μmol/L VM 26 could take effect, which showed enhanced sensitivity of the transfected strain to apoptosis agent.These results suggest that IL 6 gene may play an important role in promoting apoptosis of cancer cells and inhibiting the formation and growth of tumor.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1998年第1期20-22,64,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
全军八五重点攻关课题
