摘要
研究了六氰合亚铁酸镍修饰电极对单胺类递质及其代谢产物的催化机理，首次以该 电极为色谱电化学检测器，对８种物质进行了分离测定。同时，将微渗析活体取样技术与高 效液相色谱一电化学检测联用测定了ＳＤ大鼠脑尾核处的单胺类递质及其代谢产物，并研究了 Ｃａ２＋浓度对脑中递质的影响，拓展了电分析化学在生物活体分析中的应用范围。
In this paper, we prepared a nickel hexacyanoferrate (NiHCF) chemically modified electrode (CME) and discussed its mechanism of the electrocatalytic oxidation to monoamine transmitters and their metabolites. The separation was performed on a C18 column with an eluting system containing phosphate buffer (pH = 5. 0) and methanol. A ed linear relationship was found between the concentration and peak heights of the monoamine transmitters and their metabolites from 10-8 mol/L to 10-5 mol/L. We coupled microdialysis sampling techique with LC and determined the monoamine transmitters and their metabolites of caudatum in freely moving rat. At the same time, we also studied the effect of Ca2+ on the transimmitters.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第7期814-818,共5页
Chinese Journal of Analytical Chemistry
基金
中国科学院长春应用化学研究所电分析化学开放研究实验室资助课题
关键词
微渗析取样
大鼠脑尾核
单胺类
神经递质
测定
Nickel hexacyanoferrate, chemically modified elected, chromatography
microdialysis Sampling technique, caudatum, rat, monoamine transmitters, metabolites
