摘要
采用液体培养的方法，分离纯化了丝状真菌宛氏拟青霉（Paecilomyces variotii）并研究了其对苯并[a]葸、苯并[a]芘、苯并[b]荧葸、苯并[k]荧葸、茚并[1，2，3-cd]芘的降解效果。结果表明：接种处理30d，该菌株对混合体系中5种PAHs的降解率为16．1％～24．6％，而对单一体系中的降解率为10．4％～33．3％；同时，对单一与混合体系中PAHs的降解作用存在一定差异，苯并[k]荧蒽和苯并[b]荧蒽在单一体系中降解率增大，而其他种类的则减小。本研究结果为高环多环芳烃共代谢机理研究和多环芳烃复合污染水土环境的生物修复提供了一种新的种质资源。
Microbial degradation is a feasible treatment technology for the remediation of PAHs- contaminated soils, but its effect depends on many factors including the metabolic activity of microorganisms and the bioavailability of PAHs in contaminated soils. Many researches have fo- cused on the microbes that can degrade high molecular weight （HMW） PAHs. In this paper, a filamentous fungus Paecilomyces variotii was isolated and purified from a heavy oil-contaminated soil, and its capability in degrading HMW PAHs Benzo[ a] anthracene （BaA）, benzo [ a] pyrene （BaP）, benzol b] fluoranthene （BbF）, benzo[ k] fluoranthene （BkF）, and indeno [ 1,2, 3-cd] pyrene （IcdP） was examined with liquid culture. After 30 days inoculation with P. vari- otii, the degradation rate of test PAHs was 16. 1 %-24. 6% in mixed system and 10. 4%--33.3% in single system, and there was a definite difference in the degradability of test PAHs in the two systems. In single system, the degradation rate of benzo[ k ] fluoranthene and benzo[ b ] fluoranthene increased, while the other PAHs were in adverse. The findings of this study, could provide a new germplasm resource in the researches of the co-metabolism mechanisms of HMW PAHs and the bioremediation of soil-water environment contaminated by compound PAHs.
出处
《生态学杂志》
CAS
CSCD
北大核心
2009年第9期1842-1846,共5页
Chinese Journal of Ecology
基金
国家环境保护部项目(1440800011)
广州市环境保护局资助项目
关键词
高环多环芳烃
生物降解
真菌
high molecular weight PAHs
biodegradation
fungi.
