摘要
目的以PGenesil-1(Pg)为载体构建针对乙型肝炎病毒表面抗原(HBsAg)基因的shRNA表达载体PGenesil-1-HBs(Pgs)。方法设计、合成针对HBs区的三对DNA序列,分别插入PGenesil-1中,并对重组质粒进行限制性内切酶谱和DNA序列测定分析。结果3个不同的重组shRNA表达载体经限制性酶识别和DNA序列测定完全正确。结论成功构建了3个针对HBs基因的shRNA表达载体。
Objective To construct the shR.NA expressing vectors to target HBsAg gene of HBV using the PGenesil-1 as vector. Methods Three couples of DNA sequences targeting the HBs genes were synthesized and inserted PGenesil-1 plasmid vector,and the recombinant plasmid was analyzed by restriction enzyme digestion and DNA sequencing. Results The restriction enzyme profiles and DNA sequences of 3 recombinant shRNA expression vectors corresponded the designs. Conclusion Three shRNA expression vectors targeting HBs gene were constructed successfully.
出处
《川北医学院学报》
CAS
2009年第5期425-427,共3页
Journal of North Sichuan Medical College
