摘要
目的研究Ki-67、p53、mdm2在中耳胆脂瘤上皮细胞中的表达及其相关性,探讨其在中耳胆脂瘤发病机制中的作用。方法采用免疫组化技术检测20例后天原发性胆脂瘤中耳炎上皮组织(20耳)和8例正常外耳道皮肤上皮组织(8耳)中Ki-67、p53、mdm2蛋白的表达情况并分析其相关性。结果Ki-67在中耳胆脂瘤上皮组织中主要表达在上皮细胞的基底层、棘细胞层,颗粒层和角质层未见表达。正常外耳道皮肤中Ki-67局限表达于上皮细胞的基底层,且数量较少,分布稀疏。Ki-67在中耳胆脂瘤上皮细胞的标记指数(LI)为19.2±4.5,显著高于正常外耳道皮肤上皮细胞(6.6±2.1,t=2.247,P=0.035)。p53在中耳胆脂瘤上皮组织中主要表达于上皮细胞的基底层、棘细胞层,颗粒层和角质层散在表达。正常外耳道皮肤上皮细胞中p53未见表达或极少量表达。p53在中耳胆脂瘤上皮细胞的LI为16.5±4.1,显著高于外耳道皮肤上皮细胞(2.1±0.4,t=2.605,P=0.015)。mdm2在中耳胆脂瘤上皮组织中主要表达在上皮细胞的棘细胞层、颗粒层和角质层,在基底层细胞表达较弱。正常外耳道皮肤上皮细胞mdm2未见表达或极少量表达。mdm2蛋白在中耳胆脂瘤上皮细胞的LI为36.1±7.0,显著高于外耳道皮肤上皮细胞(1.7±0.5,t=3.325,P=0.003)。Pearson相关分析发现中耳胆脂瘤上皮细胞p53、mdm2蛋白表达存在正相关(r=0.499,P=0.031)。结论与正常外耳道皮肤上皮细胞比较,中耳胆脂瘤上皮细胞具有过度增殖的趋势。p53、mdm2异常表达与中耳胆脂瘤发生密切相关,二者在上皮细胞层的分布特性与其病理状态相关。
Objective To investigate the expressions and correlation of Ki-67, p53 and mdm2 in human middle ear cholesteatoma, and assess their potential pathogenic role in the development of middle ear cholesteatoma. Methods The protein expressions of Ki-67, p53 and mdm2 were detected with immunohistochemical methods in 20 samples of primary cholesteatoma （20 ears） and 8 control samples of skin from normal external acoustic meatus skin （8 ears）, and the correlations were then analyzed. Results In the epithelial tissues of middle ear eholesteatoma, Ki-67 was expressed mainly in the basal layer of epithelial cells and stratum spinosum, and was negative in the cells of granular layer and stratum corneum. In the epithelium of external acoustic meatus, Ki-67 locally expressed in the basal layer of epithelial cells in a little amount and sparse distribution. The labeling index （LI） of Ki67 in epithelial cells of middle ear cholesteatoma was 19. 2±4. 5, significantly higher than that in the external acoustic meatus skin （6. 6±2. 1, t=2.247, P=0. 035）. p53 expressed mainly in the basal layer of epithelial cells and stratum spinosum, sporadically expressed in the cells of granular layer and stratum corneum in the cholesteatoma epithelial tissue, and none or a little amount of p53 expressed in the epithelial cells of external acoustic meatus skin. The LI of p53 was 16.5±4. 1 in the cholesteatoma epithelium, which was significantly higher than that in the external acoustic meatus skin （2. 1± 0. 4, t=2. 605, P=0. 015）. mdm2 was expressed mainly in the cells of spinous layer, granular layer and stratum corneum. None or a little amount of mdm2 was expressed in the epithelial cells of external acoustic meatus skin. The LI of mdm2 was 36. 1 ± 7. 0 in the cholesteatoma epithelium, and it was significantly higher than that in the external acoustic meatus skin （1.7±0. 5, t=3. 325, P=0. 003）. There was positive correlation between p53 and mdm2 expression in eholesteatoma epithelium analyzed by Pearson correlation test （ r= 0. 499, P=0. 031）. Conclusions The cholesteatoma epithelium displays a tendency of over proliferation compared with the normal external acoustic meatus skin. The abnormal expression of p53 and mdm2 is closely correlated with the pathogenic mechanism of cholesteatoma.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2009年第9期1089-1092,共4页
Medical Journal of Chinese People's Liberation Army
