摘要
为探讨胶质源性神经营养因子（ＧＤＮＦ）在中枢神经系统中的表达，采用免疫组化法检测该因子在成年大鼠中枢神经系统部分区域的分布。结果发现大脑皮质各层有极弱阳性的神经元；小脑皮质分子层及蒲肯野细胞呈强阳性反应；脊髓灰质中阳性神经元广为分布，尤以前角、后角Ⅰ、Ⅱ板层及Ｃｌａｒｋ核明显。此外，脊髓胶质细胞和中央管室管膜上皮均有ＧＤＮＦ阳性信号表达，表明ＧＤＮＦ对成年大鼠中枢神经系统内的多种神经元群均有功能作用。
Glial cell linederived neurotrophic factor (GDNF) is a distant member of the transforming growth factorβ, originally isolated by virtue of its ability to induce dopamine uptake and cell survival in cultures of embryonic ventral midbrain dopaminergic neurons, and more recently it has been shown to be a potent neurotrophic factor for motorneurons. However, the distribution of this molecule in the central nervous system (CNS) is not known yet. In the present study, the expression of GDNF in the CNS of rat was investigated by using immunohistochemistry. GDNF immunoreactive fibers or bodies were found in molecular layer and Purkinje layer of cerebellum. In the spinal cord, neuronal expression was found widely, especially in ventral horn, Clarke's column. GDNF was also expressed in fiber terminals of Ⅰand Ⅱlamella of dorsal horn. Additionally, GDNA expression was found in the ependymal epithelium and neuroglia of spinal cord. The nuclei of neuroglia were stronger than perikaryon. The neurons in cerebral cortex showed very weak immunoreaction. We conclude that GDNF is expressed in many different cellular systems within the CNS, suggesting the multiple functions of GDNF in the adult CNS.
出处
《华西医科大学学报》
CSCD
1998年第3期289-291,306,共4页
Journal of West China University of Medical Sciences
关键词
中枢神经系统
大鼠
GDNF
Glial cell linederived neurotrophic factor Central nervous system Rat
