摘要
为探讨高比放活性血管活性肠肽（１２５Ｉ－ＶＩＰ）在正常小鼠体内的分布特性，取纯系昆明种小鼠３０只，随机分为６组，每组５只，在尾静脉注入１２５Ｉ－ＶＩＰ０．２ｍｌ（７４ｋＢｑ）后１８０分钟内的不同时相，将小鼠断头处死，收集血液、肺、肝、肠和肾，称重并测定其放射性，最后换算为每克组织的ＩＤ％。另取纯系昆明种小鼠３０只，分组同上，尾静脉分别注入含１０、２０和４０μｇＶＩＰ的１２５Ｉ－ＶＩＰ溶液，于５和２０分钟将小鼠断头处死，组织收集、数据处理同前。结果显示：１２５Ｉ－ＶＩＰ迅速浓聚于肺，血液清除Ｔ１／２在２０分钟内，２０分钟后肝与血液放射性无显著性差异（Ｐ＞０．０５），肠道始终处于低放射性水平，肾脏放射性随时间迅速下降。ＶＩＰ以剂量依赖方式抑制肺、肝和肠等组织对１２５Ｉ－ＶＩＰ的摄取，１０、２０和４０μｇＶＩＰ对肺摄取示踪剂的抑制率在５分钟时分别为４５．１２％、５６．６４％和６８．１２％，在２０分钟时分别为为５３．６５％、７１．３８％和７９．０３％。由此表明小鼠各组织对示踪剂的摄取具有受体介导特性；１２５Ｉ－ＶＩＰ主要被肺摄取，经肾脏排泄，肝胆系统无分泌。该生物分布特点有利于１３１Ｉ（１２５Ｉ）－ＶＩＰ显像探测胃?
This experiment was designed to investigate the biodistribution characters of 125Ivasoactive intestinal peptide(VIP) with high specific activity in normal mice and the effect of VIP on the distributive test. After intravenous injection of 125IVIP, the mice were killed during 180 min.Blood, lungs, liver, intestine and kidneys were collected respectively and measured in γ counter, finally the measurements of their radioactivity (cpm) were converted into ID%/g tissue and the results were expressed as ±s. The effect of VIP on distributive test was evaluated by simultaneously injection of 125IVIP (74kBq) which contained 10,20 and 40μg VIP respectively; the mice were killed at 5 and 20 min respectively, the collection of tissues and the management of data were the same as above. Most of the 125IVIP was distributed in the lungs rapidly, the radioactivity was mainly eliminated through kidneys; the T1/2 of activity in blood was shorter than 20 min; the difference in activity between liver and blood was not significant after 20min (P>005); the activity intestine remained lower during the experiment. The uptake of 125IVIP in lungs, liver and intestine was inhibited by VIP in dose dependence. The rates of inhibitory effectiveness of 10,20 and 40μg VIP in lungs were 45.12%,56.64% and 68.12% respectively at 5 min, and 53.65%,71.38% and 79.03% respectively at 20 min. The present study indicated that the uptake of 125IVIP in various tissues of the mice possesses the character mediated by VIP receptor, 125IVIP is mainly accumulated by lungs, eliminated through kidneys, and the hepatobiliary system cannot remove it. The biodistribution character of 125IVIP is helpful to 131Ⅰ(123Ⅰ)VIP imaging in the detection of gastrointestinal tumor.
出处
《华西医科大学学报》
CSCD
1998年第3期281-284,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
关键词
血管活性肠肽
药物分布
动物实验
胃肠道肿瘤
Vasoactive intestinal peptide Pharmacological distribution Animal experiment
