摘要
目的探讨CD44在人肝细胞癌中的行为及价值。方法应用Northernblot及RT-PCR方法对30例人肝细胞中CD44表达进行研究。结果 Northernblot结果表明,30例癌组织中有11例阳性,30例癌旁肝组织有5例阳性,CD44在肝细胞癌组织中的表达与癌周肝组织中的表达无显著差异(P>0.05)。RT-PCR研究表明,30例癌组织中有16例阳性,30例癌旁肝组织中有10例阳性,CD44在癌组织及癌旁肝组织中的表达无显著性差异(P>0.05)。癌组织中的表达与AFP及肝细胞癌的分期间存在相关关系(P=0.024;P=0.007)。结论 CD44基因可作为肝癌分期的一项指标并可能在肝癌形成过程中起调节作用。
Objective To investigate the relationship between CD44 mRNA expression and hepatocellular carcinoma (HCC). Methods CD44 mRNA expression in liver cells were assessed in 30 patients with HCC by using Northern blot hybridization and RT-PCR. Results The expression of CD44 mRNA in the 30 cases was nat significantly higher in HCC tissues than in corresponding noncancerous parachyma (P>0.05). No significant correlation between CD44 expression and grade of differentiation and HBsAg was found (P>0.05). There was a correlation between CD44 expression and AFP (P<0.05) and the clinical stage of HCC (P=0.007)。Conclusion CD44 can be used as an indicator for the stage of HCC and may play important roles in the pathogenesis and metastasis of human hepatocellular carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第5期421-422,共2页
Chinese Journal of Experimental Surgery
