摘要
通过检测甜瓜中与拟南芥韧皮部特异性表达蛋白的编码基因AtPP2相类似的基因(AtPP2-like)的表达情况,以及甜瓜植株的胼胝质沉积效应,证明了这二者与甜瓜植株对蚜虫抗性之间的联系。利用反转录PCR(RT-PCR)的方法,检测了甜瓜两个AtPP2类似蛋白的编码基因lec17和lec26,受到HrpNEa的诱导表达增强,同时通过苯胺蓝染色鉴定了胼胝质在HrpNEa处理的甜瓜植株中沉积增加。接种蚜虫后的表型观测结果表明HrpNEa处理的甜瓜生长加快,抗虫效应增加。口针刺吸活动的电波信号图(Electrical penetration graph,EPG)结果显示,HrpNEa处理的甜瓜与对照相比蚜虫的取食活动明显减少,刺探性活动增加,取食频率大幅降低。这些结果表明HrpNEa处理增强了甜瓜对蚜虫的抗性。
It has been reported that HrpNEa could induce the insect resistance of Arabidopsis. Here we demonstrated that HrpNa also showed potential to enhance the aphid repellency on Cucumis melo. The expression of AtPP2-like gene （lecl7 and lec26）in Cucumis melo determined by RT-PCR and the callose deposition in phloem measured by aniline blue staining were both induced by HrpNEa. The action of aphids after placed on Cucumis melo indicated that HrpNFa could induce insect resistance on Cucumis melo .EPG （electrical penetration graph）results revealed that nrpNEa could decrease the feeding frequency of aphids. Thus, we draw the conclusion that HrpNEa could enhance the resistance of Cucumis melo to aphids.
出处
《华北农学报》
CSCD
北大核心
2009年第4期188-192,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金(0771441)
