摘要
目的在超声生物显微镜（ｕｌｔｒａｓｏｕｎｄｂｉｏｍｉｃｒｏｓｃｏｐｙ，ＵＢＭ）眼前段成像技术基础上设计活体人眼虹膜形态定量测量方法，并对其精确性进行评价。方法在ＡｕｔｏＣＡＤＶｅｒｓｉｏｎ１２软件的基础上设计ＵＢＭ图像辅助测量方法。对ＵＢＭ实时获取的活体人眼前段不同子午线的ＰＣＸ格式图像采用该软件进行进一步处理和定量测量，包括虹膜轮廓及特征线的提取，虹膜厚度、虹膜膨隆曲率、虹膜静息长度的测量。对同一操作者对同一眼的同一子午线ＵＢＭ图像获取及同一幅图的测量结果的一致性进行评价。对不同操作者间的测量结果进行比较。采用该方法对正常人的虹膜形态进行初步定量研究。结果ＵＢＭ获取图像的变异系数小于５％。同一操作者对同一幅图各测量指标测量的变异系数均小于５％，不同操作者间部分测量结果差异有显著性，但测量结果绝对值相差不大，各测量者测量结果的变异系数相当。２１岁以上的９６例（１９２只眼）正常人虹膜形态定量测量结果：虹膜静息长度３．６９９±０．３９７ｍｍ，虹膜曲率半径９．１０１±１．４０８ｍｍ，虹膜平均厚度０．４０６±０．０４２ｍｍ。结论活体人眼虹膜形态实时定量测量方法的精确度和重复性可满足活体人眼生理、病理生理及相关疾病研?
Objective To evaluate the intraobserver and interobserver reproducibility of real time measurement of iris morphology in living human eyes. Methods Based on the platform of software (Autocad, version 12), we developed an ultrasound biomicroscopy (UBM) image assistant measuring system. Using the system, we can perform the iris configuration quantitative measurement in living eyes. The measuring parameters include: iris rest length, radius of iris curvature and the thickness of different part of iris. Ten anterior segment images of one normal individual were obtained by a single operator to evaluate the intraobserver reproducibility of image capture, and ten times of measurement of one image were performed by a single operator to assess the reproducibility of image measurement. The measurements of three independent observers were compared to investigate interobserver reproducibility in quantitative measurement. Intraobserver and interobserver reproducibility of measurement were assessed by calculating the coefficient of variation for each individual observer and by using F test to detect a difference among the observers. The iris configurations of 96 subjects (192 eyes) were measured. Results Intraobserver reproducibility ranged from 0.9～4.9% in all measured parameters. Interobserver reproducibility for some of the measured parameters varied considerably and was affect by subjective interpretation of visualized anatomic landmarks. The preliminary measured parameters in Chinese show that iris rest length is 3.699±0.397 mm, radius of iris curvature is 9.101±1.408 mm, the average thickness of iris is 0.406±0.042 mm. Conclusions The intraobserver reproducibility and the measured accuracy can fit the requisition of the ocular biometry, physiology and pharmacology studies. The method supplies a new assistance for UBM image measurement.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1998年第5期369-372,共4页
Chinese Journal of Ophthalmology
关键词
虹膜形态
图像处理
超声生物显微镜
Iris configuration Image processing, computer assisted Ultrasound biomicroscopy
