摘要
目的研究山莨菪碱对海马ＡＴＰ和ＡＴＰ酶活性的影响。方法建立沙鼠全脑缺血再灌注模型，脑缺血时间１０ｍｉｎ。动物分为假手术组，常温缺血组，常温缺血再灌注组，山莨菪组，每组８只动物。测定脑缺血后和再灌注３０及６０ｍｉｎ时海马突触体ＡＴＰ和ＡＴＰ酶活性。结果脑缺血后ＡＴＰ与缺血前后相比明显下降（Ｐ＜０．０１），常温再灌注３０ｍｉｎ时，ＡＴＰ恢复到缺血前的６４％。山莨菪碱组ＡＴＰ含量明显高于常温组（Ｐ＜０．０５）。沙鼠脑缺血１０ｍｉｎ后Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性明显降低（Ｐ＜０．０５），再灌注后Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶的活性有所恢复，但明显低于假手术组（Ｐ＜０．０５）。亚山莨菪碱组Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶的活性明显高于常温再灌注组（Ｐ＜０．０５）。结论脑缺血后山莨菪碱治疗可促进海马突触体ＡＴＰ和ＡＴＰ酶活性的恢复，这可能是其减轻脑缺血再灌注损伤的机制之一。
Objective To study the effects of anisodamine on ATP content and ATP-ase activities in the hippocampus after ischemia in gerbils.Method Cerebral ischemia/reperfusion model was established in 4 groups of gerbils (n=8 each) by clamping both carotid arteries for 10 min and then, reperfusion for 30 and 60 min to determine the synaptosomic ATP content and ATP-ase activities.Results The ATP content was evidently decreased after cerebral ischemia (P<0.01). Following 30 min of reperfusion, it was restored to 64% the pre-ischemic level, but was significantly lower than in the anisodamine therapy group (P<0.05). The activites of Na+, K+-ATPase and Ca2+-ATPase were also decreased after ischemia (P<0.05). They recovered to certain extent, but were still lower than those in the sham-operation group (P<0.01). The ATPase activities in the anisodamine group were evidently higher than in the reperfusion group (P<0.05).Conclusion Anisodamine therapy for cerebral ischemia can enhance the recovery of hippocampal synaptosomic ATP content and ATPase activities, which might be one of the mechanisms for anisodamine to alleviate reperfusion injuries.
出处
《徐州医学院学报》
CAS
1998年第4期264-266,共3页
Acta Academiae Medicinae Xuzhou
基金
江苏省卫生厅资助
关键词
脑缺血
再灌注损伤
海马
ATP
ATP酶
山莨菪碱
Cerebral ischemia Reperfusion ATP Na+,K+-ATPase Ca2+-ATPase Mild hypotheria Gerbils
