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食管鳞癌细胞HIF-1α与survivin的关系 被引量：3

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摘要 目的探讨食管鳞癌细胞Eca-109中缺氧诱导因子-1α基因(HIF-1α)与抗凋亡基因生存素survivin的关系。方法培养食管鳞癌细胞Eca-109,用氯化钴模拟缺氧以促进HIF-1α表达,再以RNA干扰抑制HIF-1α基因表达,Western blot法检测其中HIF-1α和survivin的表达。结果氯化钴培养后Eca-109细胞的HIF-1α蛋白表达增加,survivin蛋白表达也增加,并与氯化钴浓度有关;以RNA干扰方法抑制了HIF-1α基因的干扰细胞(Eca-109/shRNA)中HIF-1α蛋白表达减少,survivin蛋白表达也减少。结论在食管鳞癌细胞Eca-109中,HIF-1α基因和survivin表达具有高度的相关性,抑制HIF-1α表达可能通过抑制survivin而促进肿瘤细胞的凋亡。
作者 张捷 邹晓平 肖斌 郝波 张国新
机构地区 南京大学医学院附属鼓楼医院 南京医科大学第一附属医院
出处 《山东医药》 CAS 北大核心 2009年第32期43-44,共2页 Shandong Medical Journal
关键词 缺氧诱导因子1 Α亚基 食管肿瘤 生存素 氯化钴 细胞凋亡
分类号 R735.1 [医药卫生—肿瘤]
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参考文献7

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同被引文献20

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引证文献3

二级引证文献7

山东医药
2009年 第32期

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