摘要
本文主要研究了甘蓝型油菜幼苗对NaCl胁迫的抗氧化应答情况。结果表明,在200mmol/LNaCl胁迫下,随着胁迫时间的延长,油菜幼苗叶片的相对含水量逐渐下降,细胞内的电解质渗出物逐渐增多,电导率逐渐升高;POD、SOD和CAT三种抗氧化酶活性在开始时都逐渐提高,24h后达到最大值,随后逐渐降低。对NaCl胁迫过程中这三种抗氧化酶基因进行实时荧光定量PCR分析,分析结果显示这三种抗氧化酶基因的表达与其酶活性的变化一致。因此,NaCl胁迫诱导了POD、SOD和CAT三种抗氧化物酶基因的过量表达,抗氧化酶活性相应提高,从而在一定程度上提高了植物对NaCl胁迫的耐受能力。
Antioxidant responses to NaCl stress in Brassica rapeseed seedlings were studied. The results showed that the leaves relative water content of Brassica rapeseed was gradually decreased and the electronic conductivity was increased under 200 mmol/L NaCl solution stress. The activity ofperoxidas （POD）, superoxide dismutase （SOD） and catalase （CAT） was gradually increased during 0-24 hours under NaCl stress. After 24 hours the activity of these antioxidases was maximum and subsequently decreased. Quantitative real-time PCR analysis also indicated that the over-expressions of three antioxidant enzyme genes were induced and were consistent with the variance of their enzymatic activities,which enhanced the tolerance of Brassica rapeseed against NaCl stress to a certian extent.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2009年第4期725-729,共5页
Genomics and Applied Biology
基金
国家自然科学基金(30871555)
四川省青年科技基金(07ZQ026-002)
四川省科技基金(05SJ011-012)
四川省教育厅(2005B047)共同资助
