摘要
目的：建立反相高效液相色谱法分别测定体外释药试验接收液中吲哚美辛浓度和贴剂含药量，寻找月桂氮酮在透皮吸收制剂中的最佳浓度。方法：接收液中加入炔诺酮作为内标，经乙醚萃取后，选用ＬｉｃｈｒｏｓｏｒｂＲＰ１８（４．６ｍｍ×２５０ｍｍ，５μｍ）柱，流动相为甲醇水乙腈冰醋酸（６３∶２２∶１５∶０．１５），流速０．８ｍｌ·ｍｉｎ－１，检测波长２５４ｎｍ；贴剂先用医用汽油溶出，ｐＨ７．２磷酸缓冲液提取，再如上测定。结果：两峰分离完全，吲哚美辛线性范围０．８μｇ·ｍｌ－１～２０μｇ·ｍｌ－１，ｒ＝０．９９９８，检测限为２ｎｇ，日内偏差和日间偏差分别为０．９２％（ｎ＝５）和０．３７％（ｎ＝５），接收液和贴剂中吲哚美辛测定方法的平均回收率分别为９９．９８％（ＲＳＤ＝０．２０％）和９２．２６％（ＲＳＤ＝０．８１％）；贴剂中吲哚美辛平均含量为标示量的９２．７４％（ＲＳＤ＝１．８０％）；得月桂氮酮的最佳处方浓度为６．４６％。结论：方法灵敏。
OBJECTIVE: To discuss the methods for synthesizing perlolyrine. METHODS: Perlolyrine was synthesized from furfuryl alcohol and tryptophan (schem 1), and from furfuryl alcohol and tryptamine (scheme 2), respectively. RESULTS: The yield of synthetic perlolyrine from scheme 1 was 7%. Its chemical structure was confirmed by UV, IR, MS and NMR, and the yield of synthetic perlolyrine from scheme 2 was 12%. The chemical structure was confirmed by MS and UV. CONCLUSION: The two schemes are suitable for synthesizing perlolyrine, but scheme 2 has a good yield of synthesizing perlolyrine. It is a better way to synthesize perlolyrine.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
1998年第8期487-491,共5页
Chinese Pharmaceutical Journal
