摘要
采用Fura－2／AM荧光探剂法测定不同龄大鼠大脑皮层和海马两个脑区细胞内游离钙浓度（[Ca2+]i）以及尼莫地平对老龄大鼠（24月龄）上述两个脑区[Ca2+]i的影响，另外用同位素标记法测定尼莫地平对老龄大鼠上述两脑区细胞线粒体摄钙的影响。结果发现，随增龄两个脑区细胞[Ca2+]i呈渐进性增长，应用尼莫地平后．老龄大鼠两脑区细胞[Ca2＋]i的升高明显被抑制（P＜0．01），同时细胞线粒体摄钙能力亦明显升高（P＜0.01）但无明显剂量依赖性。提示尼莫地平可通过阻断钙通道、促进细胞内钙库对Ca2＋封存等不同途径降低神经细胞[Ca2＋]i，延缓神经细胞的损伤和老化。
The intracelluar free Ca2＋ concentration([Ca2＋]i) in cerebral cortex and hippocarnpal cells of rats in different age and the cffects of nimodiplne on aging wistar rats by using Fura-2/AM and isotopic tracer technique were studied. It was found that the [Ca2＋]i in cercbral cortex and hippocampal cells decreased markedly after treatcd with nimodipine,while the uptake of 45Ca in whtochondria was increased signifcantly. These findings implied that nimodipine might dccrease the [Ca2＋]i and alleviate neural damage caused by Ca2＋overload in aging rats through blocking calcium channels and promoting the uptake of Ca2＋ in dritochondria.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
1998年第4期238-239,共2页
Chinese Journal of Gerontology
关键词
尼莫地平
海马
大脑皮层
钙
老年鼠
Aging
Nimodipine
Fura-2/AM
Hippocampal
Mitochondria
