摘要
目的：探讨人端粒酶逆转录酶（hTERT）和生存素（survivin）蛋白在胆囊癌组织中的表达及其意义。方法：采用免疫组化S-P法对60例胆囊癌、15例胆囊腺瘤和15例慢性胆囊炎组织中hTERT和survivin蛋白的表达进行检测。结果：胆囊癌组织中hTERT和survivin的阳性表达率明显高于胆囊腺瘤组织和慢性胆囊炎组织（P〈0.01）。hTERT的阳性表达与患者性别、年龄、胆囊癌组织学类型、是否伴有结石、病理分级、Nevin临床分期、是否转移以及术后生存时间等因素均无明显关系（P〉0.05）。NevinⅢ-Ⅴ期survivin的阳性表达率高于NevinⅠ-Ⅱ期（P〈0.05）,有淋巴结或远处转移组survivin的阳性表达率高于无转移组（P〈0.05）。胆囊癌中hTERT表达与survivin表达呈正相关关系（P〈0.05）。结论：hTERT和survivin在胆囊癌中均有异常高表达且呈正相关关系,两者共同参与了胆囊癌的发生、发展过程并起一定的协同作用。hTERT过表达是胆囊癌发生中的早期事件,survivin的表达水平在一定程度上提示了胆囊癌的不良生物学行为。
Objective：To investigate the expressions and clinical significance of human telomerase reverse transcriptase（hTERT）and survivin in gallbladder carcinoma. Methods ：The expressions of hTERT and survivin in 60 cases of primary gallbladder carcinoma, 15 cases of gallbladder adenoma and 15 cases of chronic cholecystitis were determined using streptavidin-peroxidase immunohistochemical method. Results：The positive rates of hTERT and survivin were significantly higher in gallbladder carcinoma group than in gallbladder adenoma group and chronic cholecystitis group （ P 〈 0.01 ）. The positive expression of hTERT in gallbladder carcinoma had no significant correlation with the gender, age, histologic type, gallbladder stone, pathological grade, Nevin stage, metastasis or survival time after operation （P 〉 0.05）. The positive expression was significantly higher in Nevin staging Ⅲ- Ⅴ group than in Nevin staging Ⅰ - Ⅱ group （P 〈 0.05）. The positive expression of survivin was higher in lymph node or distant metastasis group than in non-metastasis group （P 〈 0.05 ）. In gallbladder carcinoma, the expression of hTERT had a positive correlation with that of survivin （ P 〈 0.05 ）. Conclusions： Abnormal high expressions of hTERT and survivin protein are observed in gallbladder carcinoma, and there is a positive correlation between them. Both hTERT and survivin play an important role in the pathogenesis and progression of gallbladder carcinoma, and may have a cooperative effect on the carcinogenesis. High expression of hTERT is an early event in the pathogenesis of gallbladder carcinoma. The overexpression of survivin indicates malignant biological behavior of gallbladder carcinoma to some extent.
出处
《蚌埠医学院学报》
CAS
2009年第8期659-662,共4页
Journal of Bengbu Medical College
基金
蚌埠医学院自然科学研究资助项目(BY0720)
关键词
胆囊肿瘤
人端粒酶逆转录酶
生存素
免疫组织化学
gallbladder neoplasms
human telomerase reverse transcriptase
survivin
immunohistochemistry
