摘要
目的比较骨碎补水、醇提取液及柚皮甙对小鼠成骨细胞MC3T3-E1基质矿化作用的影响。方法制备骨碎补的水、醇提取液。以小鼠成骨细胞株MC3T3-E1为细胞模型,用Von kossa钙化染色法了解上述药物提取液对成骨细胞基质矿化作用的影响。结果1 mg/L骨碎补水提液及0.01 mg/L骨碎补醇提液均可提高MC3T3-E1细胞体外钙化点面积百分比(P<0.05)。三组柚皮苷溶液作用组与对照组相比无明显区别。结论骨碎补水相和醇相提取物中分别存在有较高活性的促成骨细胞基质矿化的物质。
Objective To explore the effects of Drynaria fortunei J. Smith （DFS） and naringin from Drynaria fortunei J. Smith on the matrix mineralization of mouse osteoblastic cell line MC3T3-E1 cells. Methods Two DFS preparations were extracted with distilled water （DFS aqueous-extract） and 95% ethanol （DFS ethanol -extract）, respectively. A mouse osteoblast cell line MC3T3-E1 was used as a cell model for screening potency of DFS and naringin. Von kossa staining method was used to understand the effects of the two extracts and naringin from Drynaria fortunei J. Smith on the matrix mineralization of mouse osteoblastic cell line MC3T3-E1 cells. Results 1 mg/L of the aqueous-extract and 0. 01 mg/L of the ethanol-extract were able to promote the area percentage of matrix mineralization MC3T3-E1 ceils （P 〈 O. 05 ）. But it showed that naringin solutions had no effects on MC3T3-E1 cell area of matrix min- eralization. Conclusion In the DFS aqueous- and ethanol-extracts, there are some of the components to promote the activity of matrix mineralization of osteoblast.
出处
《口腔医学》
CAS
2009年第8期403-404,408,共3页
Stomatology
基金
浙江省科技厅重点科研项目(2005c23034)
关键词
中药
骨碎补
柚皮甙
成骨细胞
基质矿化
Chinese herb drugs
Drynaria fortunei J. Smith
naringin
osteoblast
matrix mineralization
