摘要
目的:探讨肿瘤浸润树突状细胞(tumor infiltration dendritic cells,TIDC)在口腔鳞癌中的活化率和意义。方法:采用免疫组织化学方法(Envision法)对10例正常口腔黏膜、10例癌前病变及30例不同分化程度口腔鳞癌中CD1a、HLA-DR的表达进行检测,并结合细胞形态特征确定树突状细胞(dendritic cells,DC)在组织中的浸润程度,依据Sprinzl等提出的分级标准分级,对其活化率进行统计分析。结果:①DC活化率:癌前病变组显著高于正常口腔黏膜组和口腔鳞癌组(P<0.01);而口腔鳞癌组略高于正常口腔黏膜组,但差异无显著性(P>0.05)。②CD1a表达阳性的DC活化率均与年龄、性别、发病部位无关(P>0.05)。③CD1a表达阳性的DC浸润程度与活化率间呈正相关(r=0.615,P<0.01)。结论:①口腔鳞癌中TIDC数量减少并存在功能缺陷,且随着恶性程度增加而加重。②TIDC浸润度及活化率反映了组织局部的免疫状况,可作为判断机体抗肿瘤免疫能力的一项指标。③肿瘤组织中TIDC浸润度及活化率降低,可能是肿瘤发生免疫逃逸的原因之一。
Objective： To investigate the activated rate and significance of tumor infiltration dendritic cells （TIDC） in oral squamous cell carcinoma （OSCC）. Methods： Normal mucosa （10 cases）, precancerous lesion （10 cases） and oral squamous cell carcinoma （30 cases） were included in this study. C1a protein and HLA-DR were examined immunohistochemically. The degree of infiltration of dendritic cells （DC） was studied according to the criteria reported by Sprinzl. Results： ①The activated rate of DC in precancerous lesion group was higher than the other two groups, but no significant difference was showed （P〉0.05）. ②The activated rate of DC did not show correlation to gender, age and site （P〉0.05）. ③Positive correlation was revealed between infiltrative degree and activated rate of DC （P〈0.01）. Conclusions： ①In OSCC, the TIDC decreased in number and dysmaturity. ②The lower density of DC infihration and activated rate in tumor tissue might reflect the microenviromeutal immunodeficiency of hosts, and might be the important index to prediction of prognosis.③The lower density of DC infiltration and activated rate may be one of the reasons for immune escape.
出处
《口腔颌面外科杂志》
CAS
2009年第4期253-256,共4页
Journal of Oral and Maxillofacial Surgery
