摘要
目的:探讨转化生长因子-β1(transforming growth factor-β1,TGF-β1)和诱导型一氧化氮合酶(inducible nitricoxide synthase,iNOS)在舌鳞癌细胞株Tca8113中的表达,为进一步研究TGF-β1和iNOS在舌鳞癌浸润转移中的作用提供实验依据。方法:应用SP免疫组化方法和逆转录-聚合酶链反应(RT-PCR)法,对舌鳞癌细胞株Tca8113中TGF-β1和iNOS的蛋白、mRNA表达情况进行检测。结果:SP免疫组化检测到TGF-β1/iNOS蛋白在舌鳞癌Tca8113细胞胞浆中呈强阳性表达,RT-PCR检测到舌鳞癌Tca8113细胞株中TGF-β1/iNOS的mRNA条带明显。结论:TGF-β1/iNOS的蛋白和mRNA在舌鳞癌Tca8113细胞株中同时呈高水平表达。
Objective： To investigate the expression level of transforming growth factor-β1 （TGF-β1）and inducible nitric oxide synthase （iNOS） in Tca8113 cells and provide basis for further study on the role of TGF-β1 and iNOS in invasion and metastasis of human tongue carcinoma. Methods： mRNA and protein of TGF-β1/iNOS in Tca8113 cells were detected by RT-PCR and SP immunohistochemistry. Results： The band of TGF-β1/iNOS mRNA were obvious, and the expression of TGF-β1/iNOS protein were strongly positive in Tca8113 cells. Conclusion： There were high level expression of TGF-β1/ iNOS in Tea8113 cells.
出处
《口腔颌面外科杂志》
CAS
2009年第4期243-247,共5页
Journal of Oral and Maxillofacial Surgery
基金
广州市医药卫生科技项目(2008-YB-203)
关键词
转化生长因子-Β1
诱导型一氧化氮合酶
舌鳞癌
TCA8113细胞
transforming growth factor-β1 （TGF-β1）
inducible nitric oxide synthase （iNOS）
tongue squamous cell carcinoma
Tca8113 cells
