摘要
建立了血清中丙戊酸的高效液相色谱快速分析法。用ＺｏｒｂａｘＯＤＳ柱，ｐＨ４．２硫酸水溶液为流动相，检测波长２１０ｎｍ，流速０．８ｍＬ／ｍｉｎ，线性范围０．８２μｍｏｌ／Ｌ～１．８ｍｍｏｌ／Ｌ（ｒ＝０．９９６），最低检测限０．８２μｍｏｌ／Ｌ，平均回收率为９８．３２％±２．２７％，日内与日间的变异系数分别为３．３１％（ｎ＝５）和４．８２％（ｎ＝７）。
A method for the determination of valproic acid level in serum with high performance liquid chromatography was established. The conditions were Zorbax ODS (250mm×4.6mm, 5μm) column, 0.8mL/min sulfuric acid solution (pH 4.2) eluent and UV 210nm detector. The linear range was 0.82μmol/L to 1 8μmol/L (r=0.996) and the detection limit was 0.82μmol/L. The average recovery was 98.32%±2 27% and the coefficients of variation were 3.31% (n=5) and 4.82% (n=7) for within day and day to day tests respectively. This direct method was compared with the derivatization method.
出处
《色谱》
CAS
CSCD
北大核心
1998年第4期365-366,共2页
Chinese Journal of Chromatography
关键词
丙戊酸
血浓度
人血清
HPLC
测定
high performance liquid chromatography, valproic acid, serum concentration
