摘要
目的:研究马勃多糖的单糖组成及其物质的量之比。方法:马勃多糖经三氟醋酸水解,糖腈乙酰酯衍生化后,利用GC-MS法分析单糖组成,相对校正因子法测定单糖的物质的量之比。结果:马勃的三种多糖CGPⅠ-1、CGP-Ⅱ和CGP-Ⅲ均含有甘露糖、葡萄糖和半乳糖,各单糖对应物质的量之比分别为1.22∶11.28∶3.92,1.74∶26.45∶0.36,1.06∶9.90∶3.29。此外,CGP-Ⅲ还含有一种未知单糖,由于无相应标糖无法确定其成分。结论:马勃的三种多糖均由多种单糖构成。Rtx-5silms柱可用于马勃多糖的单糖组成分析。
Objective： To identify the components of polysaccharides from Calvatia gigantea and their molar ratio. Methods： The components of the polysaccharide were identified by gas chromatography - mass spectrometry （GC - MS）. Monosaceharides from polysaceharides were derivatized to acetylated aldononitriles before determination. Results： Polysaccharide were composed of CGPI - Ⅰ, CGP - Ⅱ and CGP - Ⅲ, all including mannose, glucose and galactose, with the molar ratio of 1.22：11.28：3.92,1.74：26.45：0.36, 1.06：9.9：3.29 in different CGPs. Besides; an unknown monosaecharide was found in CGP- Ⅲ. Conclusion： CGP is made up of several monosaccharides. The column of Rtx - 5silms is eligible for the analysis of Calvatia gigantea oolvsaccharides.
出处
《长治医学院学报》
2009年第4期254-256,共3页
Journal of Changzhi Medical College
关键词
马勃
多糖
GC—MS
Calvatia gigantea
Polysaccharides
Gas chromatography - mass spectrometry （GC - MS）
