摘要
目的:体外培养大鼠胚胎脊髓神经干细胞,观察其生长、增殖和分化特点。方法:利用倒置显微镜、MTT法、免疫细胞化学等方法检测神经干细胞的增殖、分化情况。结果:分离的脊髓神经干细胞可形成大量悬浮的神经球,并可进行体外扩增传代,神经球内的细胞均呈nestin阳性。在添加10%胎牛血清7d后,神经球细胞可分化为有神经元、星形胶质细胞特异性抗原表达的细胞。结论:体外培养大鼠胚胎脊髓可得到大量神经干细胞,并能分化为神经元和星形胶质细胞。
Objective： To isolate and culture neural stem cells from rat embroyonic spinal cord and observe the characteristics during growth, generation and differentiation. Methods： We detected the generation and differentiation of neural stem ceils by light microscope, MTT test and the methods of immunocytochemistry. Results： Cells separated from spinal cord of embryonic rats could proliferate and passage in vitro. Nestin-positive cells were detected in neural sphere. The separated cells cultured in serum with 10% FBS for 7 days might naturally differentiate into cells which strongly expressed neurons or astroeytes antigen at some rate. Conclusion： The above results suggest that there are neural stem cells from rat embroyonie spinal cord, which may differentiate neurons and astrocytes.
出处
《中国医药导报》
CAS
2009年第25期24-26,共3页
China Medical Herald
基金
山东省中青年科学家奖励基金(2006BS03023)
关键词
大鼠
脊髓
神经干细胞
细胞培养
Rats
Spinal cord
Neural stem cells
Cell culture
